Beltran A, Caro E, Marcé R M, Cormack P A G, Sherrington D C, Borrull F
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Marcel.lí Domingo s/n, Campus Sescelades, 43007 Tarragona, Spain.
Anal Chim Acta. 2007 Jul 30;597(1):6-11. doi: 10.1016/j.aca.2007.06.040. Epub 2007 Jun 23.
A molecularly imprinted polymer (MIP) designed to enable the selective extraction of carbamazepine (CBZ) from effluent wastewater and urine samples has been synthesised using a non-covalent molecular imprinting approach. The MIP was evaluated chromatographically in the first instance and its affinity for CBZ also confirmed by solid-phase extraction (SPE). The optimal conditions for SPE consisted of conditioning of the cartridge using acidified water purified from a Milli-Q system, loading of the sample under basic aqueous conditions, clean-up using acetonitrile and elution with methanol. The attractive molecular recognition properties of the MIP gave rise to good CBZ recoveries (80%) when 100 mL of effluent water spiked with 1 microg L(-1) was percolated through the polymer. For urine samples, 2 mL samples spiked with 2.5 microg L(-1) CBZ were extracted with a recovery of 65%. For urine, the linear range was 0.05-24 mg L(-1), the limit of detection was 25 microg L(-1) and precision, expressed as relative standard deviation at 0.5 mg L(-1) (n=3), was 3.1% and 12.6% for repeatability and reproducibility between days, respectively.
采用非共价分子印迹方法合成了一种分子印迹聚合物（MIP），用于从废水和尿液样本中选择性萃取卡马西平（CBZ）。首先对该MIP进行了色谱评估，其对CBZ的亲和力也通过固相萃取（SPE）得到了证实。SPE的最佳条件包括：使用从Milli-Q系统纯化的酸化水对柱进行预处理，在碱性水溶液条件下上样，用乙腈进行净化，并用甲醇洗脱。当100 mL加标1 μg L⁻¹ 的废水通过该聚合物渗滤时，MIP具有吸引力的分子识别特性使得CBZ的回收率良好（80%）。对于尿液样本，加标2.5 μg L⁻¹ CBZ的2 mL样本经萃取后的回收率为65%。对于尿液，线性范围为0.05 - 24 mg L⁻¹，检测限为25 μg L⁻¹，精密度以0.5 mg L⁻¹ 时的相对标准偏差表示（n = 3），日内重复性为3.1%，日间重现性为12.6%。
