Arad Ayala, Nammouz Sherin, Nov Yuval, Ohel Gonen, Bejar Jacob, Vadasz Zahava
Departments of Pathology (A.A, J.B.)Obstetrics and Gynecology (S.N., G.O.)Division of Allergy and Clinical Immunology (Z.V.), Bnai Zion Medical CenterBruce Rappaport Faculty of Medicine (A.A., G.O., J.B., Z.V.), Technion Israel Institution of TechnologyDepartment of Statistics (Y.N.), University of Haifa, Haifa, Israel.
Int J Gynecol Pathol. 2017 Jan;36(1):42-49. doi: 10.1097/PGP.0000000000000283.
Preeclampsia (PET) is a hypertensive disorder that affects 2% to 8% of pregnant women. Recent observations support the hypothesis that upregulation of placental anti-angiogenic factors are responsible for the clinical manifestations of the disease. Neuropilin-1 (NP-1) is a transmembrane protein that acts as a coreceptor for vascular endothelial growth factor and as a regulatory protein in the immune system. The aim of the study was to evaluate the expression of NP-1 in PET and normal placentas. Nineteen placental specimens from severe PET pregnancies were compared with 20 placental specimens of women with low-risk pregnancy. All the specimens underwent immunohistochemical staining with anti-human NP-1 antibody. The degree of NP-1 staining was measured both for intensity and extent. Our study demonstrated NP-1 immunoreactivity mainly in the decidual cells, the intermediate trophoblast, and the syncytiotrophoblast, particularly in the areas in the syncytial knots and shed particles. The particles were strongly NP-1 immunoreactive. The expression of NP-1 in the syncytiotrophoblast was lower in placentas of PET compared with control (P=0.017). Shedding of syncytiotrophoblast particles from placenta to maternal blood occurs in normal pregnancies and is enhanced during PET and contributes to the maternal vascular injury that characterizes PET. Our new observation that shows strong NP-1 immunoreactivity of these particles, and decreased NP1 expression in syncytiotrophoblast of PET placentas in comparison to the control group, may imply a role of NP-1 in PET.
子痫前期（PET）是一种高血压疾病，影响2%至8%的孕妇。最近的观察结果支持这样一种假说，即胎盘抗血管生成因子的上调是该疾病临床表现的原因。神经纤毛蛋白-1（NP-1）是一种跨膜蛋白，它作为血管内皮生长因子的共受体，并在免疫系统中作为一种调节蛋白。本研究的目的是评估NP-1在PET胎盘和正常胎盘中的表达。将19例重度PET妊娠的胎盘标本与20例低风险妊娠女性的胎盘标本进行比较。所有标本均用抗人NP-1抗体进行免疫组织化学染色。对NP-1染色的强度和范围进行测量。我们的研究表明，NP-1免疫反应主要存在于蜕膜细胞、中间滋养层细胞和合体滋养层细胞中，特别是在合体结节和脱落颗粒区域。这些颗粒具有强烈的NP-1免疫反应性。与对照组相比，PET胎盘的合体滋养层细胞中NP-1的表达较低（P=0.017）。在正常妊娠中，合体滋养层颗粒从胎盘向母体血液的脱落会发生，并且在PET期间会增强，这有助于PET所特有的母体血管损伤。我们的新观察结果显示这些颗粒具有强烈的NP-1免疫反应性，并且与对照组相比，PET胎盘的合体滋养层细胞中NP1表达降低，这可能意味着NP-1在PET中发挥作用。
