Maneesai Putcharawipa, Prasarttong Patoomporn, Bunbupha Sarawoot, Kukongviriyapan Upa, Kukongviriyapan Veerapol, Tangsucharit Panot, Prachaney Parichat, Pakdeechote Poungrat
Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Faculty of Medical Sciences, Nakhonratchasima College, Nakhonratchasima 30000, Thailand.
Nutrients. 2016 Feb 29;8(3):122. doi: 10.3390/nu8030122.
This study examined the effect of Carthamus tinctorius (CT) extract plus captopril treatment on blood pressure, vascular function, nitric oxide (NO) bioavailability, oxidative stress and renin-angiotensin system (RAS) in N(ω)-Nitro-l-arginine methyl ester (l-NAME)-induced hypertension. Rats were treated with l-NAME (40 mg/kg/day) for five weeks and given CT extract (75 or 150 or 300 or 500 mg/kg/day): captopril (5 mg/kg/day) or CT extract (300 mg/kg/day) plus captopril (5 mg/kg/day) for two consecutive weeks. CT extract reduced blood pressure dose-dependently, and the most effective dose was 300 mg/kg/day. l-NAME-induced hypertensive rats showed abnormalities including high blood pressure, high vascular resistance, impairment of acetylcholine-induced vasorelaxation in isolated aortic rings and mesenteric vascular beds, increased vascular superoxide production and plasma malondialdehyde levels, downregulation of eNOS, low level of plasma nitric oxide metabolites, upregulation of angiotensin II type 1 receptor and increased plasma angiotensin II. These abnormalities were alleviated by treatment with either CT extract or captopril. Combination treatment of CT extract and captopril normalized all the abnormalities found in hypertensive rats except endothelial dysfunction. These data indicate that there are synergistic antihypertensive effects of CT extract and captopril. These effects are likely mediated by their anti-oxidative properties and their inhibition of RAS.
本研究考察了红花提取物加卡托普利治疗对N（ω）-硝基-L-精氨酸甲酯（L-NAME）诱导的高血压大鼠血压、血管功能、一氧化氮（NO）生物利用度、氧化应激和肾素-血管紧张素系统（RAS）的影响。大鼠连续五周给予L-NAME（40mg/kg/天），并连续两周给予红花提取物（75或150或300或500mg/kg/天）：卡托普利（5mg/kg/天）或红花提取物（300mg/kg/天）加卡托普利（5mg/kg/天）。红花提取物剂量依赖性地降低血压，最有效的剂量为300mg/kg/天。L-NAME诱导的高血压大鼠表现出包括高血压、高血管阻力、离体主动脉环和肠系膜血管床中乙酰胆碱诱导的血管舒张受损、血管超氧化物生成增加和血浆丙二醛水平升高、内皮型一氧化氮合酶（eNOS）下调、血浆一氧化氮代谢产物水平降低、血管紧张素II 1型受体上调和血浆血管紧张素II增加等异常。这些异常通过红花提取物或卡托普利治疗得到缓解。红花提取物和卡托普利联合治疗使高血压大鼠中发现的所有异常（内皮功能障碍除外）恢复正常。这些数据表明红花提取物和卡托普利具有协同降压作用。这些作用可能是由它们的抗氧化特性及其对RAS的抑制作用介导的。
