Maneesai Putcharawipa, Iampanichakul Metee, Chaihongsa Nisita, Poasakate Anuson, Potue Prapassorn, Rattanakanokchai Siwayu, Bunbupha Sarawoot, Chiangsaen Petcharat, Pakdeechote Poungrat
Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Antioxidants (Basel). 2021 Mar 27;10(4):523. doi: 10.3390/antiox10040523.
In this study, we examine whether Linn. (CT) can prevent Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced cardiac and vascular dysfunction in rats. Male Sprague Dawley rats were given L-NAME (40 mg/kg, drinking water) and orally administered with CT extract (300 mg/kg/day) or lisinopril (2.5 mg/kg/day) for 5 weeks. The main phytochemical components of the CT extract were found to be flavonoids. The CT extract alleviated the high blood pressure in rats receiving L-NAME. Decreased vasorelaxation responses to acetylcholine and enhanced contractile responses to sympathetic nerve stimulation in aortic rings and mesenteric vascular beds of L-NAME treated rats were ameliorated by CT extract supplementation. Left ventricular hypertrophy and dysfunction were developed in L-NAME rats, which were partially prevented by CT extract treatment. The CT extract alleviated upregulated endothelial nitric oxide synthase expression, decreased plasma nitrate/nitrite levels, and increased oxidative stress in L-NAME rats. It suppressed high levels of serum angiotensin-converting enzyme activity, plasma angiotensin II, and cardiac angiotensin II type 1 receptor, NADPH oxidases 2, nuclear factor-kappa B, and tumor necrosis factor-alpha expression. The CT extract, therefore, partially prevented L-NAME-induced hypertension and cardiovascular alterations in rats. These effects might be related to a reduction in the oxidative stress and renin-angiotensin system activation due to L-NAME in rats.
在本研究中，我们检测了[植物名称未给出]（CT）是否能预防盐酸Nω-硝基-L-精氨酸甲酯（L-NAME）诱导的大鼠心脏和血管功能障碍。雄性Sprague Dawley大鼠给予L-NAME（40 mg/kg，饮用水），并口服CT提取物（300 mg/kg/天）或赖诺普利（2.5 mg/kg/天），持续5周。发现CT提取物的主要植物化学成分是黄酮类化合物。CT提取物减轻了接受L-NAME的大鼠的高血压。补充CT提取物改善了L-NAME处理大鼠主动脉环和肠系膜血管床对乙酰胆碱的血管舒张反应降低以及对交感神经刺激的收缩反应增强的情况。L-NAME大鼠出现左心室肥厚和功能障碍，CT提取物治疗可部分预防。CT提取物减轻了L-NAME大鼠中内皮型一氧化氮合酶表达上调、血浆硝酸盐/亚硝酸盐水平降低以及氧化应激增加的情况。它抑制了高水平的血清血管紧张素转换酶活性、血浆血管紧张素II以及心脏血管紧张素II 1型受体、NADPH氧化酶2、核因子-κB和肿瘤坏死因子-α的表达。因此，CT提取物部分预防了L-NAME诱导的大鼠高血压和心血管改变。这些作用可能与降低大鼠中由L-NAME引起的氧化应激和肾素-血管紧张素系统激活有关。
