Tianjin Key Laboratory of Composite and Functional Materials, School of Material Science and Engineering, Tianjin University , Tianjin 300072, China.
Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Department of Neurosurgery, Tianjin Medical University General Hospital and Key Laboratory of Neurotrauma, Variation, and Regeneration, Ministry of Education and Tianjin Municipal Government , Tianjin 300052, China.
ACS Nano. 2016 Mar 22;10(3):3323-33. doi: 10.1021/acsnano.5b06939. Epub 2016 Mar 7.
Exosomes are a class of naturally occurring nanoparticles that are secreted endogenously by mammalian cells. Clinical applications for exosomes remain a challenge because of their unsuitable donors, low scalability, and insufficient targeting ability. In this study, we developed a dual-functional exosome-based superparamagnetic nanoparticle cluster as a targeted drug delivery vehicle for cancer therapy. The resulting exosome-based drug delivery vehicle exhibits superparamagnetic behavior at room temperature, with a stronger response to an external magnetic field than individual superparamagnetic nanoparticles. These properties enable exosomes to be separated from the blood and to target diseased cells. In vivo studies using murine hepatoma 22 subcutaneous cancer cells showed that drug-loaded exosome-based vehicle delivery enhanced cancer targeting under an external magnetic field and suppressed tumor growth. Our developments overcome major barriers to the utility of exosomes for cancer application.
外泌体是一类内源性分泌的天然纳米颗粒，存在于哺乳动物细胞中。由于外泌体的供体不合适、可扩展性低和靶向能力不足，其临床应用仍然是一个挑战。在这项研究中，我们开发了一种基于外泌体的双功能超顺磁纳米颗粒簇，作为癌症治疗的靶向药物传递载体。所得到的基于外泌体的药物传递载体在室温下表现出超顺磁性，对外磁场的响应比单个超顺磁纳米颗粒更强。这些特性使外泌体能够从血液中分离出来并靶向病变细胞。使用小鼠肝癌 22 皮下癌细胞的体内研究表明，载药的基于外泌体的载体递药在外磁场下增强了癌症靶向性，并抑制了肿瘤生长。我们的研究成果克服了外泌体在癌症应用中的主要障碍。
