Sun Kun, Yang Fan, Kong Yingjun, Kang Jiyao, Cao Wei, Yang Xiaoyan, Zha Shenghua, Zhang Guifeng, Wang Minglin
Sheng Wu Gong Cheng Xue Bao. 2015 Nov;31(11):1660-8.
A method for quantitation of collagen was established by detecting marker peptide with high performance liquid chromatography-mass spectrometry (HPLC-MS). Theoretical marker peptides were selected by sequence comparison. Bovine collagen type I was digested with trypsin. Marker peptides typical for collagen type I were identified with HPLC-MS. The relationship between the abundance of marker peptides and collagen concentration was established. The results show that GEAGPSGPAGPTGAR and the other 5 peptides showed high resolution during chromatographic separation and high signal intensity during MS analysis. Peptide signal intensity and collagen concentration showed a good linear relationship in the range from 0.1 to 3 mg/mL. Bovine tendon and collagen sponge were used as actual samples and collagen contents were determined as 90.2% and 93.4% respectively. Quantitation of marker peptides of collagen was a feasible method to identify and quantify collagens in medical device research and development.
通过高效液相色谱-质谱联用（HPLC-MS）检测标记肽，建立了一种胶原蛋白定量方法。通过序列比对选择理论标记肽。用胰蛋白酶消化牛I型胶原蛋白。用HPLC-MS鉴定I型胶原蛋白的典型标记肽。建立了标记肽丰度与胶原蛋白浓度之间的关系。结果表明，GEAGPSGPAGPTGAR和其他5种肽在色谱分离过程中具有高分辨率，在质谱分析中具有高信号强度。肽信号强度与胶原蛋白浓度在0.1至3mg/mL范围内呈良好的线性关系。以牛肌腱和胶原海绵为实际样品，测定胶原蛋白含量分别为90.2%和93.4%。胶原蛋白标记肽的定量是医疗器械研发中鉴定和定量胶原蛋白的一种可行方法。
