Kumazawa Yuki, Taga Yuki, Iwai Kenji, Koyama Yoh-Ichi
Japan Institute of Leather Research , 520-11 Kuwabara, Toride, Ibaraki 302-0017, Japan.
Nippi Research Institute of Biomatrix , 520-11 Kuwabara, Toride, Ibaraki 302-0017, Japan.
J Agric Food Chem. 2016 Aug 3;64(30):6051-7. doi: 10.1021/acs.jafc.6b02132. Epub 2016 Jul 20.
Identification of the animal source of leather is difficult using traditional methods, including microscopic observation and PCR. In the present study, a LC-MS method was developed for detecting interspecies differences in the amino acid sequence of type I collagen, which is a major component of leather, among six animals (cattle, horse, pig, sheep, goat, and deer). After a dechroming procedure and trypsin digestion, six tryptic peptides of type I collagen were monitored by LC-MS in multiple reaction monitoring mode for the animal source identification using the patterns of the presence or absence of the marker peptides. We analyzed commercial leathers from various production areas using this method, and found some leathers in which the commercial label disagreed with the identified animal source. Our method enabled rapid and simple leather certification and could be applied to other animals whether or not their collagen sequences are available in public databases.
使用传统方法（包括显微镜观察和聚合酶链式反应）很难鉴定皮革的动物来源。在本研究中，开发了一种液相色谱 - 质谱法，用于检测六种动物（牛、马、猪、羊、山羊和鹿）皮革的主要成分I型胶原蛋白氨基酸序列中的种间差异。经过脱铬处理和胰蛋白酶消化后，通过液相色谱 - 质谱以多反应监测模式监测I型胶原蛋白的六种胰蛋白酶肽，利用标记肽的有无模式进行动物来源鉴定。我们使用这种方法分析了来自不同产地的商业皮革，发现一些皮革的商业标签与鉴定出的动物来源不一致。我们的方法能够实现快速、简单的皮革认证，并且无论其他动物的胶原蛋白序列是否可在公共数据库中获取，都可应用于这些动物。
