Jiang Huijiao, Su Yan, Shen Yu
Wei Sheng Wu Xue Bao. 2015 Oct 4;55(10):1343-9.
To compare the antigenicity, adhesion inhibition activity and immune protection of the serum immunized with recombinant adhesins Clumping factor A (CfA), Fibronection binding protein A-A ( FnBPA-A) and Fibronection binding protein A-BCD (FnBPA-BCD) of Staphylococcus aureus.
ClfA, FnBPA-A and FnBPA-BCD genes were expressed and the resulting recombinant proteins were induced and purified. After immunizing mice with these purified proteins in combination or alone, the serum antibodies of mice were analyzed and compared for their antigenicity, adhesion inhibition activity and immune protection.
The recombinant FnBPA-BCD protein had better fibronection (Fn) binding ability than that of fibrinogen (Fg) binding and ClfA. FnBPA-A had better binding capacity to Fg than that of FnBPA-BCD. The antibody titer induced by ClfA and FnBPA-A were better than that of FnBPA-BCD, and three proteins co-immunization group had better antibody titer and adhesion inhibition to Staphylococcus aureus than those of single recombinant protein immunization group (P < 0.05). The immune protection rate for both ClfA and three proteins co-immunization group were 100% and for FnBPA-A and FnBPA-BCD immunization group the immune protection rate were 80% and 50%, respectively.
Our data suggest that coimmunization with these 3 recombinant proteins helps to achieve better immune effect.
比较用金黄色葡萄球菌重组黏附素聚集因子A（CfA）、纤连蛋白结合蛋白A-A（FnBPA-A）和纤连蛋白结合蛋白A-BCD（FnBPA-BCD）免疫的血清的抗原性、黏附抑制活性和免疫保护作用。
表达ClfA、FnBPA-A和FnBPA-BCD基因，并诱导和纯化产生的重组蛋白。用这些纯化蛋白单独或联合免疫小鼠后，分析并比较小鼠血清抗体的抗原性、黏附抑制活性和免疫保护作用。
重组FnBPA-BCD蛋白与纤连蛋白（Fn）的结合能力优于与纤维蛋白原（Fg）的结合能力及ClfA。FnBPA-A与Fg的结合能力优于FnBPA-BCD。ClfA和FnBPA-A诱导的抗体效价优于FnBPA-BCD，三种蛋白联合免疫组的抗体效价和对金黄色葡萄球菌的黏附抑制作用均优于单一重组蛋白免疫组（P<0.05）。ClfA和三种蛋白联合免疫组的免疫保护率均为100%，FnBPA-A和FnBPA-BCD免疫组的免疫保护率分别为80%和50%。
我们的数据表明，这三种重组蛋白联合免疫有助于获得更好的免疫效果。
