Czajkowska-Mysłek Anna, Siekierko Urszula, Gajewska Magdalena
Department of Food Quality, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, 36 Rakowiecka, 02-532, Warsaw, Poland.
Department of Meat and Fat Technology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology, 36 Rakowiecka, 02-532, Warsaw, Poland.
Lipids. 2016 Apr;51(4):413-21. doi: 10.1007/s11745-016-4133-1. Epub 2016 Mar 3.
The aim of this study was to develop a simple method for simultaneous determination of selected cis/cis PUFA-LNA (18:2), ALA (18:3), GLA (18:3), EPA (20:5), and DHA (22:6) by silver ion high-performance liquid chromatography coupled to a diode array detector (Ag-HPLC-DAD). The separation was performed on three Luna SCX Silver Loaded columns connected in series maintained at 10 °C with isocratic elution by 1% acetonitrile in n-hexane. The applied chromatographic system allowed a baseline separation of standard mixture of n-3 and n-6 fatty acid methyl esters containing LNA, DHA, and EPA and partial separation of ALA and GLA positional isomers. The method was validated by means of linearity, precision, stability, and recovery. Limits of detection (LOD) for considered PUFA standard solutions ranged from 0.27 to 0.43 mg L(-1). The developed method was used to evaluate of n-3 and n-6 fatty acids contents in plant and fish softgel oil capsules, results were compared with reference GC-FID based method.
本研究的目的是开发一种通过银离子高效液相色谱联用二极管阵列检测器（Ag-HPLC-DAD）同时测定选定的顺式/顺式多不饱和脂肪酸 - 亚麻酸（18:2）、α-亚麻酸（18:3）、γ-亚麻酸（18:3）、二十碳五烯酸（EPA，20:5）和二十二碳六烯酸（DHA，22:6）的简单方法。分离在三根串联的Luna SCX银负载柱上进行，柱温保持在10°C，用1%乙腈的正己烷等度洗脱。所应用的色谱系统能够对含有亚麻酸、DHA和EPA的n-3和n-6脂肪酸甲酯标准混合物进行基线分离，并对α-亚麻酸和γ-亚麻酸的位置异构体进行部分分离。该方法通过线性、精密度、稳定性和回收率进行了验证。所考虑的多不饱和脂肪酸标准溶液的检测限（LOD）范围为0.27至0.43 mg L⁻¹。所开发的方法用于评估植物软胶囊油和鱼油软胶囊中的n-3和n-6脂肪酸含量，并将结果与基于气相色谱 - 火焰离子化检测器（GC-FID）的参考方法进行比较。
