Denev Roumen V, Kuzmanova Ivalina S, Momchilova Svetlana M, Nikolova-Damyanova Boryana M
Bulgarian Academy of Sciences, Institute of Organic Chemistry with Center of Phytochemistry, Acad. G. Bontchev St, bl. 9, Sofia 1113, Bulgaria.
J AOAC Int. 2011 Jan-Feb;94(1):4-8.
A silver ion HPLC procedure is described that is suitable to determine the fatty acid composition of plant seed oils. After conversion of fatty acids to p-methoxyphenacyl derivatives, it was possible to achieve baseline resolution of all fatty acid components with 0 to 3 double bonds, including the positionally isomeric 18:1 fatty acids oleic acid (cis 9-18:1), petroselinic acid (cis 6-18:1), and cis-vaccenic acid (cis 11-18:1), in aniseed oil (Pimpinella anisum, Apiaceae) by a single gradient run on a single cation exchange column laboratory converted to the silver ion form. The UV detector response (280 nm) was linearly related to the fatty acid concentration in the range 0.01 to 3.5 mg/mL.
描述了一种银离子高效液相色谱法，该方法适用于测定植物种子油的脂肪酸组成。在将脂肪酸转化为对甲氧基苯甲酰衍生物后，通过在实验室转化为银离子形式的单一阳离子交换柱上进行单次梯度洗脱，能够实现所有含0至3个双键的脂肪酸组分的基线分离，包括茴香籽油（茴芹属植物茴香，伞形科）中的位置异构体18:1脂肪酸油酸（顺式9-18:1）、岩芹酸（顺式6-18:1）和顺式-异油酸（顺式11-18:1）。紫外检测器响应（280 nm）在0.01至3.5 mg/mL范围内与脂肪酸浓度呈线性关系。
