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TRI6和TRI10在禾谷镰刀菌中通过cAMP信号传导调控脱氧雪腐镰刀菌烯醇(DON)产生的过程中发挥不同作用。

TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum.

作者信息

Jiang Cong, Zhang Chengkang, Wu Chunlan, Sun Panpan, Hou Rui, Liu Huiquan, Wang Chenfang, Xu Jin-Rong

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, Northwestern A&F University, Yangling, Shaanxi, 712100, China.

Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA.

出版信息

Environ Microbiol. 2016 Nov;18(11):3689-3701. doi: 10.1111/1462-2920.13279. Epub 2016 Apr 21.

DOI:10.1111/1462-2920.13279
PMID:26940955
Abstract

The biosynthesis of mycotoxin deoxynivalenol (DON) in Fusarium graminearum is regulated by two pathway-specific transcription factors Tri6 and Tri10 and affected by various host and environmental factors. In this study, we showed that cyclic adenosine monophosphate (cAMP) treatment induced DON production by stimulating TRI gene expression and DON-associated cellular differentiation in F. graminearum. Interestingly, exogenous cAMP had no effects on the tri6 mutant but partially recovered the defect of tri10 mutant in DON biosynthesis. Although the two cAMP phosphodiesterase genes PDE1 and PDE2 had overlapping functions in vegetative growth, conidiation, sexual reproduction and plant infection, deletion of PDE2 but not PDE1 activated intracellular PKA activities and increased DON production. Whereas the tri6 pde2 mutant failed to produce DON, the tri10 pde2 double mutant produced a significantly higher level of DON than the tri10 mutant. Cellular differentiation associated with DON production was stimulated by exogenous cAMP or deletion of PDE2 in both tri10 and tri6 mutants. These data indicate that TRI6 is essential for the regulation of DON biosynthesis by cAMP signalling but elevated PKA activities could partially bypass the requirement of TRI10 for TRI gene-expression and DON production, and Pde2 is the major cAMP phosphodiesterase to negatively regulate DON biosynthesis in F. graminearum.

摘要

禾谷镰刀菌中霉菌毒素脱氧雪腐镰刀菌烯醇(DON)的生物合成受两个途径特异性转录因子Tri6和Tri10调控,并受多种宿主和环境因素影响。在本研究中,我们发现环磷酸腺苷(cAMP)处理通过刺激禾谷镰刀菌中TRI基因表达和与DON相关的细胞分化来诱导DON产生。有趣的是,外源性cAMP对tri6突变体没有影响,但部分恢复了tri10突变体在DON生物合成中的缺陷。虽然两个cAMP磷酸二酯酶基因PDE1和PDE2在营养生长、分生孢子形成、有性生殖和植物感染中具有重叠功能,但缺失PDE2而非PDE1可激活细胞内PKA活性并增加DON产生。虽然tri6 pde2突变体无法产生DON,但tri10 pde2双突变体产生的DON水平明显高于tri10突变体。外源性cAMP或缺失PDE2均可刺激tri10和tri6突变体中与DON产生相关的细胞分化。这些数据表明,TRI6对于cAMP信号调节DON生物合成至关重要,但升高的PKA活性可部分绕过TRI10对TRI基因表达和DON产生的需求,并且Pde2是禾谷镰刀菌中负向调节DON生物合成的主要cAMP磷酸二酯酶。

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