Ge Jinwen, Zhai Huanchen, Tang Lei, Zhang Shuaibing, Lv Yangyong, Ma Pingan, Wei Shan, Zhou Yu, Wu Xiaofu, Lei Yang, Zhao Fengguang, Hu Yuansen
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Microorganisms. 2024 Oct 20;12(10):2093. doi: 10.3390/microorganisms12102093.
Fusarium head blight in wheat is mainly caused by and results in significant economic losses. Coenzyme Q (CoQ) is ubiquitously produced across organisms and functions as a hydrogen carrier in energy metabolism. While UbiH in serves as a hydroxylase in CoQ biosynthesis, its role in phytopathogenic fungi is not well understood. This study explored the role of the hydroxylase FgUbiH in . Using a deletion mutant, we observed reduced hyphal growth, conidial production, germination, toxin synthesis, and pathogenicity compared to the wild-type. A transcriptome analysis indicated 's involvement in regulating carbohydrate and amino acid metabolism. Deletion of impaired mitochondrial function, reducing adenosine triphosphate synthesis and increasing reactive oxygen species. Additionally, genes related to terpene skeleton synthesis and aldehyde dehydrogenase were downregulated. Our results underscore the importance of FgUbiH in 's growth, toxin production, and energy metabolism, aiding in the development of strategies for disease management.
小麦赤霉病主要由[未提及具体病因]引起，并导致重大经济损失。辅酶Q（CoQ）在所有生物中普遍产生，在能量代谢中作为氢载体发挥作用。虽然[未提及具体物种]中的UbiH在CoQ生物合成中作为羟化酶，但它在植物病原真菌中的作用尚不清楚。本研究探讨了羟化酶FgUbiH在[未提及具体物种]中的作用。使用[未提及具体名称]缺失突变体，我们观察到与野生型相比，菌丝生长、分生孢子产生、萌发、毒素合成和致病性均降低。转录组分析表明[未提及具体名称]参与调节碳水化合物和氨基酸代谢。[未提及具体名称]的缺失损害了线粒体功能，减少了三磷酸腺苷的合成并增加了活性氧。此外，与萜类骨架合成和醛脱氢酶相关的基因被下调。我们的结果强调了FgUbiH在[未提及具体名称]的生长、毒素产生和能量代谢中的重要性，有助于制定病害管理策略。
