Najafi Maryam, Kordi-Tamandani Dor Mohammad, Arish Mohammad
Departement of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
Department of Ophthalmology, Al-Zahra Eye Hospital, Zahedan University of Medical Sciences, Zahedan 98167-43463, Iran.
J Ophthalmol. 2016;2016:5431021. doi: 10.1155/2016/5431021. Epub 2016 Jan 28.
Purpose. Pterygium is a serious eye problem in countries with high exposure to UV. However, despite numerous studies, the molecular etiology of pterygium is unclear. Recent studies have indicated that LATS1 and LATS2 genes are involved in DDR signaling pathways against continuous UV exposure. Our aim was to evaluate the LATS1 and LATS2 promoter methylation with the risk of pterygium formation. Methods. We evaluated the promoter methylation status of LATS1 and LATS2 using methylation-specific PCR technique. Also, mRNA expression of LATS1 and LATS2 was assessed in 14 cases of pterygium and 14 normal specimens by real-time PCR. Results. Promoter methylation of LATS1 and LATS2 was detected significantly between pterygium tissues and normal tissues [LATS1; OR = 4.9; 95% CI: 1.54 to 15.48, P = 0.003; LATS2; OR = 7.1; 95% CI: 1.53 to 33.19, P = 0.004]. The gene expression analysis showed a statistically significant difference between pterygium tissues and healthy controls for both LATS1 and LATS2 (P < 0.05). Conclusions. The data of this study is the first report regarding the effect of promoter methylation of the LATS1 and LATS2 in the pterygium. To confirm these data, doing further studies in various genetic populations with large sample sizes using advanced molecular techniques is proposed.
目的。在紫外线暴露量高的国家，翼状胬肉是一个严重的眼部问题。然而，尽管有大量研究，但翼状胬肉的分子病因仍不清楚。最近的研究表明，LATS1和LATS2基因参与了针对持续紫外线暴露的DDR信号通路。我们的目的是评估LATS1和LATS2启动子甲基化与翼状胬肉形成风险的关系。方法。我们使用甲基化特异性PCR技术评估LATS1和LATS2的启动子甲基化状态。此外，通过实时PCR在14例翼状胬肉病例和14个正常标本中评估LATS1和LATS2的mRNA表达。结果。在翼状胬肉组织和正常组织之间显著检测到LATS1和LATS2的启动子甲基化[LATS1；OR = 4.9；95% CI：1.54至15.48，P = 0.003；LATS2；OR = 7.1；95% CI：1.53至33.19，P = 0.004]。基因表达分析显示，翼状胬肉组织与健康对照之间LATS1和LATS2均存在统计学显著差异（P < 0.05）。结论。本研究数据是关于LATS1和LATS2启动子甲基化对翼状胬肉影响的首次报告。为证实这些数据，建议使用先进分子技术在各种遗传人群中进行大样本量的进一步研究。
