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睑板腺上皮细胞分化中的Notch信号通路

Notch Signaling in Meibomian Gland Epithelial Cell Differentiation.

作者信息

Gidfar Sanaz, Afsharkhamseh Neda, Sanjari Sara, Djalilian Ali R

出版信息

Invest Ophthalmol Vis Sci. 2016 Mar;57(3):859-65. doi: 10.1167/iovs.15-18319.

DOI:10.1167/iovs.15-18319
PMID:26943148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4782822/
Abstract

PURPOSE

Notch1 was previously shown to play a critical role in murine meibomian gland function and maintenance. In this study, we have examined the expression and activation of Notch pathway in human meibomian gland epithelial cells in vitro.

METHODS

An immortalized human meibomian gland epithelial cell (HMGEC) line was cultured under proliferative and differentiative conditions. Expression of Notch receptors and ligands were evaluated by quantitative PCR and Western blot. The effect of Notch inhibition and induction on oil production was also assessed.

RESULTS

Human meibomian gland epithelial cell expressed Notch1, Notch2, Notch3, Jagged1, Jagged2, Delta-like 1, and Delta-like 3. The level of cleaved (activated) Notch1 strongly increased with differentiation. The expression of Notch3 was inversely correlated with proliferation. Induction and inhibition of Notch1 led to an increase and decrease in the amount of oil production, respectively.

CONCLUSIONS

Notch signaling appears to play an important role in human meibomian gland epithelial differentiation and oil production. This may provide a potential therapeutic pathway for treating meibomian gland dysfunction.

摘要

目的

先前研究表明Notch1在小鼠睑板腺功能及维持中起关键作用。在本研究中，我们检测了体外培养的人睑板腺上皮细胞中Notch信号通路的表达及激活情况。

方法

将永生化人睑板腺上皮细胞（HMGEC）系在增殖和分化条件下培养。通过定量PCR和蛋白质印迹法评估Notch受体和配体的表达。还评估了Notch抑制和诱导对油脂分泌的影响。

结果

人睑板腺上皮细胞表达Notch1、Notch2、Notch3、Jagged1、Jagged2、Delta样1和Delta样3。切割（激活）的Notch1水平随分化而显著升高。Notch3的表达与增殖呈负相关。Notch1的诱导和抑制分别导致油脂分泌量增加和减少。

结论

Notch信号通路似乎在人睑板腺上皮分化和油脂分泌中起重要作用。这可能为治疗睑板腺功能障碍提供一条潜在的治疗途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/971bc40eb28f/i1552-5783-57-3-859-f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/b7bfa2af3e2e/i1552-5783-57-3-859-f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/45b784f60d90/i1552-5783-57-3-859-f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/2d81b5be69f5/i1552-5783-57-3-859-f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/dec9ed71916b/i1552-5783-57-3-859-f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/971bc40eb28f/i1552-5783-57-3-859-f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/b7bfa2af3e2e/i1552-5783-57-3-859-f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/45b784f60d90/i1552-5783-57-3-859-f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/2d81b5be69f5/i1552-5783-57-3-859-f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/dec9ed71916b/i1552-5783-57-3-859-f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed0e/4782822/971bc40eb28f/i1552-5783-57-3-859-f05.jpg

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