Cornea Division, Stein Eye Institute, University of California, Los Angeles, CA, 90095, USA.
David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
Sci Rep. 2019 Jul 17;9(1):10373. doi: 10.1038/s41598-019-46793-6.
Notch signaling has been shown to regulate the homeostasis and wound healing of the corneal epithelium. We investigated the effect of Notch inhibition in the human limbal stem/progenitor cells (LSCs) in vitro by using small molecules. Treatment of the LSCs with DAPT and SAHM1 reduced the proliferation rate and maintained the undifferentiated state of the LSCs in a concentration dependent manner. Stratification and differentiation of the corneal epithelium were not reduced after Notch inhibition, indicating that the function of the corneal basal cells is retained. Our findings suggest that Notch signaling plays a role in the proliferation and maintenance of LSCs.
Notch 信号通路已被证明可调节角膜上皮的稳态和伤口愈合。我们通过使用小分子研究了 Notch 抑制对体外人角膜缘干细胞/祖细胞（LSCs）的影响。用 DAPT 和 SAHM1 处理 LSCs 可降低增殖率，并以浓度依赖的方式维持 LSCs 的未分化状态。Notch 抑制后角膜上皮的分层和分化并未减少，表明角膜基底细胞的功能得以保留。我们的研究结果表明，Notch 信号通路在 LSCs 的增殖和维持中发挥作用。
