利比里亚高度流行疟疾地区保护性抗恶性疟原虫子孢子抗体的年龄依赖性出现情况。

Age-dependent occurrence of protective anti-Plasmodium falciparum sporozoite antibodies in a holoendemic area of Liberia.

作者信息

Hollingdale M R, Hogh B, Petersen E, Wirtz R A, Bjorkmann A

机构信息

Biomedical Research Institute, Rockville, Maryland.

出版信息

Trans R Soc Trop Med Hyg. 1989 May-Jun;83(3):322-4. doi: 10.1016/0035-9203(89)90488-4.

DOI:10.1016/0035-9203(89)90488-4

PMID:2694466

Abstract

Antibodies to Plasmodium falciparum sporozoites were detected in children aged 6 months to 9 years, and in adults, in a holendemic village near Yekepa, Liberia, by enzyme linked immunosorbent assay (ELISA) using the recombinant circumsporozoite protein R32tet32 or by inhibition of sporozoite invasion (ISI) of hepatoma cells. Both assays were significantly correlated with each other and showed that anti-sporozoite antibodies increase with age. ISI was more sensitive than ELISA and demonstrated significantly increased anti-sporozoite antibodies at age 5-6 years, when young children show partial clinical resistance to malaria. These results suggest that anti-sporozoite antibodies, as measured by ISI, may contribute to protection against malaria.

摘要

在利比里亚耶克帕附近的一个高度流行疟疾的村庄，采用酶联免疫吸附测定法（ELISA），利用重组环子孢子蛋白R32tet32，或者通过抑制疟原虫子孢子对肝癌细胞的侵袭（ISI），在6个月至9岁的儿童以及成人中检测到了恶性疟原虫子孢子抗体。两种检测方法相互之间具有显著相关性，且均显示抗子孢子抗体随年龄增长而增加。ISI比ELISA更敏感，并且显示在5至6岁时抗子孢子抗体显著增加，此时幼儿对疟疾表现出部分临床抵抗力。这些结果表明，通过ISI测定的抗子孢子抗体可能有助于预防疟疾。