Kızmaz Muhammet, Marakoğlu Kamile, Kıyıcı Aysel, Ay Emre
Department of Family Medicine, Selcuk University Medical Faculty, Konya/Turkey.
Department of Family Medicine, Selcuk University Medical Faculty, Konya/Turkey.
Clin Biochem. 2016 Jul;49(10-11):832-4. doi: 10.1016/j.clinbiochem.2016.02.011. Epub 2016 Mar 2.
Netrin-1 is a laminin like protein that has recently been suggested to involve in the pathophysiology of atherosclerosis and treatment targets. The relationship between netrin-1 and atherosclerosis has not been evaluated in a human research previously and the effect of smoking on plasma netrin-1 levels as well. In this study, we aimed to evaluate plasma netrin-1 levels in smokers and non-smokers and the impact of smoking on netrin-1 levels. Our second aim was to evaluate the existence of peripheral artery disease in these groups by means of ankle brachial index (ABI) measurements and the relation between plasma netrin-1 levels and peripheral artery disease.
Smoker and non-smoker male patients were contributed in the study, 112 males in each group. ABI of all patients was determined with an automatic device and plasma netrin-1 levels were quantified with an enzyme-linked immunosorbent assay. Netrin-1 levels were compared between smoker and non-smoker cases, and the cases with and without peripheral arterial disease.
Plasma netrin-1 levels of smokers (4.54±2.87pg/mL) were significantly higher than in non-smokers (3.81±1.26pg/mL) (p=0.015). Similarly, netrin-1 levels of cases with peripheral arterial disease (6.21±2.16pg/mL) were significantly higher than the ones without peripheral arterial disease (4.07±2.16pg/mL) (p=0.002). Amount of smoking (packet⋅year) and ABI were found to be significantly related with netrin-1 levels. The relationship between PAD and netrin-1 levels remained significant after adjusting for smoking status.
We can conclude that plasma netrin-1 levels significantly increase in smokers and peripheral artery disease is significantly related with netrin-1 levels after adjusting for smoking status.
Netrin-1是一种类层粘连蛋白，最近有人提出它参与动脉粥样硬化的病理生理过程及治疗靶点。此前尚未在人体研究中评估Netrin-1与动脉粥样硬化之间的关系，也未评估吸烟对血浆Netrin-1水平的影响。在本研究中，我们旨在评估吸烟者和非吸烟者的血浆Netrin-1水平以及吸烟对Netrin-1水平的影响。我们的第二个目的是通过踝臂指数（ABI）测量评估这些组中周围动脉疾病的存在情况以及血浆Netrin-1水平与周围动脉疾病之间的关系。
吸烟者和非吸烟者男性患者参与了本研究，每组112名男性。所有患者的ABI用自动装置测定，血浆Netrin-1水平用酶联免疫吸附测定法定量。比较吸烟者和非吸烟者病例以及有和无周围动脉疾病病例的Netrin-1水平。
吸烟者的血浆Netrin-1水平（4.54±2.87pg/mL）显著高于非吸烟者（3.81±1.26pg/mL）（p = 0.015）。同样，有周围动脉疾病病例的Netrin-1水平（6.21±2.16pg/mL）显著高于无周围动脉疾病病例（4.07±2.16pg/mL）（p = 0.002）。发现吸烟量（包·年）和ABI与Netrin-1水平显著相关。在调整吸烟状态后，PAD与Netrin-1水平之间的关系仍然显著。
我们可以得出结论，吸烟者的血浆Netrin-1水平显著升高，并且在调整吸烟状态后，周围动脉疾病与Netrin-1水平显著相关。
