Ay Emre, Marakoğlu Kamile, Kizmaz Muhammet, Ünlü Ali
Department of Family Medicine, Medical Faculty, Selcuk University, Konya, Turkey.
Department of Biochemistry, Faculty of Medicine, Selcuk University, Konya, Turkey.
J Clin Lab Anal. 2016 Nov;30(6):972-977. doi: 10.1002/jcla.21965. Epub 2016 Apr 13.
Netrin-1 is a diagnostic biomarker that has been identified recently for chronic renal failure (CRF) in animal experiments. Netrin-1 levels in urine have been shown to have increased significantly at the acute kidney damage. Human studies on the relation between the CRF and plasma netrin-1 levels have not been found in the literature. This study aimed to investigate whether plasma netrin-1 levels increased in the early stages of the CRF in diabetic patients.
Plasma samples from healthy volunteers and diabetic patients with and without microalbuminuria were collected after receiving consent. Netrin-1 in plasma was quantified by enzyme-linked immunosorbent assay and the data were analyzed to determine whether plasma netrin-1 correlates significantly with disease progression.
Plasma netrin-1 level in microalbuminuric diabetic patients was significantly higher than in normoalbuminuric diabetic patients and the control group. However, no significant difference between normoalbuminuric patients and control group was determined. Plasma netrin-1 level was significantly associated with albuminuria and estimated glomerular filtration rate, independently of age and sex.
This study supports that plasma netrin-1 level increases significantly when glomerular damage occurs in diabetic nephropathy.
Netrin-1是一种诊断生物标志物，最近在动物实验中被确定与慢性肾衰竭（CRF）有关。在急性肾损伤时，尿中Netrin-1水平已被证明显著升高。文献中尚未发现关于CRF与血浆Netrin-1水平之间关系的人体研究。本研究旨在调查糖尿病患者CRF早期血浆Netrin-1水平是否升高。
在获得同意后，收集健康志愿者以及有和无微白蛋白尿的糖尿病患者的血浆样本。采用酶联免疫吸附测定法对血浆中的Netrin-1进行定量，并对数据进行分析，以确定血浆Netrin-1是否与疾病进展显著相关。
微量白蛋白尿糖尿病患者的血浆Netrin-1水平显著高于正常白蛋白尿糖尿病患者和对照组。然而，正常白蛋白尿患者与对照组之间未发现显著差异。血浆Netrin-1水平与白蛋白尿和估计肾小球滤过率显著相关，与年龄和性别无关。
本研究支持糖尿病肾病发生肾小球损伤时血浆Netrin-1水平显著升高。
