Gwarda Radosław Ł, Aletańska-Kozak Monika, Klimek-Turek Anna, Ziajko-Jankowska Agnieszka, Matosiuk Dariusz, Dzido Tadeusz H
Department of Physical Chemistry, Chair of Chemistry, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
Chair and Department of Synthesis and Chemical Technology of Pharmaceutical Substances, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
J Chromatogr A. 2016 Apr 1;1440:229-239. doi: 10.1016/j.chroma.2016.02.065. Epub 2016 Feb 24.
One of the main problems related to chromatography of peptides concerns adverse interactions of their strong basic groups with free silanol groups of the silica based stationary phase. Influence of type and concentration of ion-pairing regents on peptide retention in reversed-phase high-performance liquid chromatography (RP-HPLC) systems has been discussed before. Here we present influence of these mobile phase additives on retention of some peptide standards in high-performance thin-layer chromatography (HPTLC) systems with C18 silica-based adsorbents. We prove, that due to different characteristic of adsorbents used in both techniques (RP HPLC and HPTLC), influence of ion-pairing reagents on retention of basic and/or amphoteric compounds also may be quite different. C18 silica-based HPTLC adsorbents provide more complex mechanism of retention and should be rather considered as mixed-mode adsorbents.
与肽的色谱分析相关的主要问题之一是其强碱性基团与硅胶基固定相的游离硅醇基团之间的不利相互作用。离子对试剂的类型和浓度对反相高效液相色谱（RP-HPLC）系统中肽保留的影响此前已有讨论。在此，我们展示了这些流动相添加剂对使用基于C18硅胶吸附剂的高效薄层色谱（HPTLC）系统中一些肽标准品保留的影响。我们证明，由于两种技术（RP-HPLC和HPTLC）中使用的吸附剂特性不同，离子对试剂对碱性和/或两性化合物保留的影响也可能有很大差异。基于C18硅胶的HPTLC吸附剂提供了更复杂的保留机制，应更多地被视为混合模式吸附剂。
