Murphy W S, Metcalfe J, Dhindsa D S, Hoversland A S
Respir Physiol. 1978 Mar;32(3):293-8. doi: 10.1016/0034-5687(78)90117-2.
In five adult opossums (Didelphis virginiana), blood hemoglobin concentration was lowered from 12.3g% to 6.4g% by daily bleeding. Blood P50 rose significantly (P is less than 0.05) from 44.5 mm Hg to 47.4 mm Hg and the concentration of 2, 3-diphosphoglycerate (DPG) increased from 14.17 mumol/g Hb to 20.90 mumol/g Hb. The observed shift in blood oxygen affinity was of similar magnitude to that recorded in humans with a similar degree of anemia, but the concomitant rise in DPG concentration was greater in opossums than in humans. In the opossum the shift in P50 with anemia can be attributed entirely to the action of DPG as a non diffusible intraerythrocytic anion.
在五只成年负鼠（弗吉尼亚负鼠）中，通过每日放血使血液血红蛋白浓度从12.3g%降至6.4g%。血液P50从44.5mmHg显著升高（P小于0.05）至47.4mmHg，2,3-二磷酸甘油酸（DPG）浓度从14.17μmol/g Hb增加至20.90μmol/g Hb。观察到的血液氧亲和力变化幅度与患相似程度贫血的人类记录值相似，但负鼠中伴随的DPG浓度升高幅度大于人类。在负鼠中，贫血时P50的变化完全可归因于DPG作为一种不可扩散的红细胞内阴离子的作用。
