MgATP对Ca2+激活的肌纤维在两个频率下测得的硬度的影响。
Effectof MgATP on stiffness measured at two frequencies in Ca2+-activated muscle fibers.
作者信息
Kawai M, Brandt P W
出版信息
Proc Natl Acad Sci U S A. 1977 Sep;74(9):4073-5. doi: 10.1073/pnas.74.9.4073.
Abstract
The stiffness of skinned crayfish single muscle fibers was continuously monitored at two frequencies. The length of the fibers was oscillated by the sum of two sine waves (5 Hz and 100 Hz) of small amplitudes. In saline containing saturating amounts of Ca2+, the stiffness ratio (5 Hz:100Hz) was constant as the MgATP (substrate) concentration was raised from 0 to 2 mu M, then it decreased with a further increment in MgATP. The systematic decrease in the stiffness ratio in MgATP above 2 mu M indicates the presence of faster transitions in the cross-bridge cycle. This dependence of the stiffness ratio on MgATP is predictable if we use the two-state model of A. F. Huxley (1957) with a modification, in which MgATP promotes the dissociation of the attached cross-bridges.
摘要
对去表皮的小龙虾单肌纤维的刚度在两个频率下进行了连续监测。纤维的长度由两个小振幅正弦波（5赫兹和100赫兹）之和进行振荡。在含有饱和量Ca2+的盐溶液中，随着MgATP（底物）浓度从0增加到2微摩尔，刚度比（5赫兹：100赫兹）保持恒定，然后随着MgATP的进一步增加而降低。在MgATP浓度高于2微摩尔时刚度比的系统性降低表明横桥循环中存在更快的转变。如果我们使用A.F.赫胥黎（1957年）的双态模型并进行修改，即MgATP促进附着横桥的解离，那么刚度比对MgATP的这种依赖性是可以预测的。
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