Kołodziejczyk Izabela, Dzitko Katarzyna, Szewczyk Rafał, Posmyk Małgorzata M
Department of Ecophysiology and Plant Development, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland.
Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland.
J Plant Physiol. 2016 Apr 1;193:47-56. doi: 10.1016/j.jplph.2016.01.012. Epub 2016 Feb 20.
Melatonin (MEL; N-acetyl-5-methoxytryptamine) plays an important role in plant stress defense. Various plant species rich in this indoleamine have shown a higher capacity for stress tolerance. Moreover, it has great potential for plant biostimulation, is biodegradable and non-toxic for the environment. All this indicates that our concept of seed enrichment with exogenous MEL is justified. This work concerns the effects of corn (Zea mays L.) seed pre-sowing treatments supplemented with MEL. Non-treated seeds (nt), and those hydroprimed with water (H) or with MEL solutions 50 and 500 μM (HMel50, HMel500) were compared. Positive effects of seed priming are particularly apparent during germination under suboptimal conditions. The impact of MEL applied by priming on seed protein profiles during imbibition/germination at low temperature has not been investigated to date. In order to identify changes in the corn seed proteome after applying hydropriming techniques, purified protein extracts of chilling stressed seed embryos (14 days, 5°C) were separated by two-dimensional electrophoresis. Then proteome maps were graphically and statistically compared and selected protein spots were qualitatively analyzed using mass spectrometry techniques and identified. This study aimed to analyze the priming-induced changes in maize embryo proteome and at identifying priming-associated and MEL-associated proteins in maize seeds subjected to chilling. We attempt to explain how MEL expands plant capacity for stress tolerance.
褪黑素（MEL；N-乙酰-5-甲氧基色胺）在植物应激防御中发挥着重要作用。各种富含这种吲哚胺的植物物种表现出更高的胁迫耐受能力。此外，它在植物生物刺激方面具有巨大潜力，可生物降解且对环境无毒。所有这些都表明我们用外源褪黑素富集种子的概念是合理的。这项工作涉及用褪黑素对玉米（Zea mays L.）种子进行播种前处理的效果。比较了未处理的种子（nt）以及用水（H）或50和500 μM褪黑素溶液（HMel50、HMel500）进行水引发处理的种子。种子引发的积极作用在次优条件下的萌发过程中尤为明显。迄今为止，尚未研究通过引发施加的褪黑素对低温吸胀/萌发过程中种子蛋白质谱的影响。为了确定应用水引发技术后玉米种子蛋白质组的变化，通过二维电泳分离了冷胁迫种子胚（14天，5°C）的纯化蛋白质提取物。然后对蛋白质组图谱进行图形和统计比较，并使用质谱技术对选定的蛋白质斑点进行定性分析和鉴定。本研究旨在分析引发诱导的玉米胚蛋白质组变化，并鉴定受冷害的玉米种子中与引发相关和与褪黑素相关的蛋白质。我们试图解释褪黑素如何扩展植物的胁迫耐受能力。
