Amorim Christiani A, Moya Cristina Fortuño, Donnez Jacques, Dolmans Marie-Madeleine
Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, bte B1.52.02, 1200, Brussels, Belgium.
Society for Research into Infertility, Brussels, Belgium.
J Assist Reprod Genet. 2016 May;33(5):617-626. doi: 10.1007/s10815-016-0681-9. Epub 2016 Mar 5.
Baboons are commonly utilized as an animal model for studies of human reproduction. However, folliculogenesis in this species has not been fully documented. The aim of this study was to assess follicle morphometry and expression of essential proteins involved in folliculogenesis in baboons.
Ovaries were recovered from four adult baboons and processed for histological evaluation and immunohistochemical analyses. Follicle proportion, follicle and oocyte diameter, theca layer thickness, number of granulosa cells, and follicle density were calculated. Immunohistochemical staining was also carried out for connexin 43 (Cx43), aromatase, and zona pellucida 3 (ZP3).
A total of 2221 follicles were counted and measured. Proportions of primordial, primary, secondary, small antral, and large antral follicles were 49, 26, 23, 1, and 1 %, respectively. The increase in follicle diameter was due not only to the increase in oocyte diameter but also to granulosa cell proliferation. Almost all antral follicles were positive for Cx43 (89.8 %), aromatase (84.8 %), and ZP3 (100 %). Most secondary follicles were positive for Cx43 (65 %) and ZP3 (64.5 %), and some primary follicles were positive only for Cx43. No primordial follicles stained positive in any of these immunohistochemical analyses. Only antral follicles showed aromatase activity.
On the basis of these results, we can conclude that folliculogenesis in baboons appears to be similar to that in humans, and this animal therefore constitutes a valuable model.
狒狒常被用作人类生殖研究的动物模型。然而，该物种的卵泡发生尚未得到充分记录。本研究的目的是评估狒狒卵泡发生过程中卵泡形态测量以及参与卵泡发生的关键蛋白的表达情况。
从四只成年狒狒体内获取卵巢，进行组织学评估和免疫组织化学分析。计算卵泡比例、卵泡和卵母细胞直径、卵泡膜层厚度、颗粒细胞数量以及卵泡密度。还对连接蛋白43（Cx43）、芳香化酶和透明带3（ZP3）进行了免疫组织化学染色。
共计数并测量了2221个卵泡。原始卵泡、初级卵泡、次级卵泡、小腔卵泡和大腔卵泡的比例分别为49%、26%、23%、1%和1%。卵泡直径的增加不仅归因于卵母细胞直径的增加，还归因于颗粒细胞的增殖。几乎所有腔卵泡的Cx43（89.8%）、芳香化酶（84.8%）和ZP3（100%）均呈阳性。大多数次级卵泡的Cx（65%）和ZP3（64.5%）呈阳性，一些初级卵泡仅Cx43呈阳性。在这些免疫组织化学分析中，没有原始卵泡呈阳性染色。只有腔卵泡显示出芳香化酶活性。
基于这些结果，我们可以得出结论，狒狒的卵泡发生似乎与人类相似，因此这种动物构成了一个有价值的模型。
