昆虫病原真菌球孢白僵菌在感染鳞翅目幼虫过程中基因的体内表达
In vivo expression of genes in the entomopathogenic fungus Beauveria bassiana during infection of lepidopteran larvae.
作者信息
Galidevara Sandhya, Reineke Annette, Koduru Uma Devi
机构信息
Department of Botany, Andhra University, Visakhapatnam, 530003 AP, India.
Hochschule Geisenheim University, Department of Phytomedicine, Von-Lade-Str. 1, D-65366 Geisenheim, Germany.
出版信息
J Invertebr Pathol. 2016 May;136:32-4. doi: 10.1016/j.jip.2016.03.002. Epub 2016 Mar 2.
The entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin is commercially available as a bio insecticide. The expression of three genes previously identified to have a role in pathogenicity in in vitro studies was validated in vivo in three lepidopteran insects infected with B. bassiana. Expression of all three genes was observed in all the tested insects starting from 48 or 72h to 10d post infection corroborating their role in pathogenicity. We suggest that it is essential to test the expression of putative pathogenicity genes both in vitro and in vivo to understand their role in different insect species.
昆虫病原真菌球孢白僵菌（Beauveria bassiana (Bals.) Vuillemin）作为一种生物杀虫剂已投入商业使用。之前在体外研究中鉴定出的三个与致病性相关的基因，在感染球孢白僵菌的三种鳞翅目昆虫体内得到了验证。从感染后48或72小时到10天，在所有测试昆虫中均观察到这三个基因的表达，证实了它们在致病性方面的作用。我们认为，为了解假定的致病性基因在不同昆虫物种中的作用，在体外和体内测试其表达至关重要。
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