Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun, 130033, Jilin Province, PR China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, PR China.
Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun, 130033, Jilin Province, PR China.
Fungal Biol. 2023 Mar;127(3):958-967. doi: 10.1016/j.funbio.2023.02.003. Epub 2023 Feb 20.
Polymycoviridae is a recently established family of mycoviruses. Beauveria bassiana polymycovirus 4 (BbPmV-4) was previously reported. However, the effect of the virus on host fungus B. bassiana was not clarified. Here, a comparison between virus-free and virus-infected isogenic lines of B. bassiana revealed that BbPmV-4 infection of B. bassiana changes morphology and could lead to decreases in conidiation and increases in virulence against Ostrinia furnacalis larvae. The differential expression of genes between virus-free and virus-infected strains was compared by RNA-Seq and was consistent with the phenotype of B. bassiana. The enhanced pathogenicity may be related to the significant up-regulation of genes encoding mitogen activated protein kinase, cytochrome P450, and polyketide synthase. The results enable studies of the mechanism of interaction between BbPmV-4 and B. bassiana.
多瘤病毒科是一个最近建立的真菌病毒科。白僵菌多瘤病毒 4（BbPmV-4）此前已有报道。然而，该病毒对宿主真菌白僵菌的影响尚不清楚。在这里，对无病毒和感染病毒的白僵菌同基因系进行了比较，结果表明，BbPmV-4 感染白僵菌改变了形态，可导致产孢减少，对玉米螟幼虫的毒力增加。通过 RNA-Seq 比较无病毒和感染病毒菌株之间的基因差异表达，与白僵菌的表型一致。致病性增强可能与丝裂原活化蛋白激酶、细胞色素 P450 和聚酮合酶基因的显著上调有关。这些结果使我们能够研究 BbPmV-4 与白僵菌之间相互作用的机制。
