Shu Xiu-Lin, Shi Qing-Shan, Feng Jin, Yang Yun-Hua, Zhou Gang, Li Wen-Ru
Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China.
Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China
J Biomater Appl. 2016 Jul;31(1):102-11. doi: 10.1177/0885328216632444. Epub 2016 Mar 4.
A series biodegradable poly (γ-glutamic acid)/beta-tricalcium phosphate (γ-PGA/TCP) nanocomposites were prepared which were composed of poly-γ-glutamic acid polymerized in situ with β-tricalcium phosphate and physiochemically characterized as bone graft substitutes. The particle size via dynamic light scattering, the direct morphological characterization via transmission electron microscopy and field emission scanning electron microscope, which showed that γ-PGA and β-TCP were combined compactly at 80℃, and the γ-PGA/TCP nanocomposites had homogenous and nano-sized grains with narrow particle size distributions. The water uptake and retention abilities, in vitro degradation properties, cytotoxicity in the simulated medium, and protein release of these novel γ-PGA/TCP composites were investigated. Cell proliferation in composites was nearly twice than β-TCP when checked in vitro using MC3T3 cell line. We also envision the potential use of γ-PGA/TCP systems in bone growth factor or orthopedic drug delivery applications in future bone tissue engineering applications. These observations suggest that the γ-PGA/TCP are novel nanocomposites with great potential for application in the field of bone tissue engineering.
制备了一系列可生物降解的聚（γ-谷氨酸）/β-磷酸三钙（γ-PGA/TCP）纳米复合材料，该复合材料由聚γ-谷氨酸与β-磷酸三钙原位聚合而成，并对其进行了物理化学表征，作为骨移植替代物。通过动态光散射测定粒径，通过透射电子显微镜和场发射扫描电子显微镜进行直接形态表征，结果表明γ-PGA和β-TCP在80℃下紧密结合，γ-PGA/TCP纳米复合材料具有均匀的纳米级晶粒，粒径分布狭窄。研究了这些新型γ-PGA/TCP复合材料的吸水和保水能力、体外降解性能、在模拟培养基中的细胞毒性以及蛋白质释放。当使用MC3T3细胞系进行体外检测时，复合材料中的细胞增殖几乎是β-TCP的两倍。我们还设想了γ-PGA/TCP系统在未来骨组织工程应用中用于骨生长因子或骨科药物递送的潜在用途。这些观察结果表明，γ-PGA/TCP是具有在骨组织工程领域应用巨大潜力的新型纳米复合材料。
