Park Sung-Bin, Hasegawa Urara, van der Vlies André J, Sung Moon-Hee, Uyama Hiroshi
a Department of Applied Chemistry , Graduate School of Engineering, Osaka University , 2-1 Yamadaoka, Suita , Osaka 565-0871 , Japan.
J Biomater Sci Polym Ed. 2014;25(17):1875-90. doi: 10.1080/09205063.2014.953404. Epub 2014 Sep 2.
A hybrid monolith of poly(γ-glutamic acid) and hydroxyapatite (PGA/HAp monolith) was prepared via biomineralization and used as a macroporous cell scaffold in bone tissue engineering. The PGA monolith having a bimodal pore size distribution was used as a substrate to induce biomineralization. The PGA/HAp monolith was obtained by immersing the PGA monolith in simulated body fluid. Pretreatment with CaCl2 enhanced the apatite-forming ability of the PGA monolith. Murine osteoblastic MC3T3-E1 cells efficiently attached and proliferated on the PGA/HAp monolith. MTT assay showed that both the PGA and PGA/HAp monolith did not have apparent cytotoxicity. Moreover, the PGA and PGA/HAp monoliths adsorbed bone morphogenetic protein-2 (BMP-2) by electrostatic interaction which was slowly released in the medium during cell culture. The PGA/HAp monolith enhanced BMP-2 induced alkaline phosphatase activity compared to the PGA monolith and a polystyrene culture plate. Thus, these PGA/HAp monoliths may have potential in bone tissue engineering.
通过生物矿化制备了聚（γ-谷氨酸）与羟基磷灰石的混合整体材料（PGA/HAp整体材料），并将其用作骨组织工程中的大孔细胞支架。具有双峰孔径分布的PGA整体材料用作诱导生物矿化的底物。通过将PGA整体材料浸入模拟体液中获得PGA/HAp整体材料。用CaCl2预处理增强了PGA整体材料的磷灰石形成能力。小鼠成骨细胞MC3T3-E1在PGA/HAp整体材料上有效附着并增殖。MTT分析表明PGA和PGA/HAp整体材料均无明显细胞毒性。此外，PGA和PGA/HAp整体材料通过静电相互作用吸附骨形态发生蛋白-2（BMP-2），在细胞培养过程中BMP-2在培养基中缓慢释放。与PGA整体材料和聚苯乙烯培养板相比，PGA/HAp整体材料增强了BMP-2诱导的碱性磷酸酶活性。因此，这些PGA/HAp整体材料在骨组织工程中可能具有潜力。
