Perez Ana P S, Biancardi Manoel F, Caires Cássia R S, Falleiros Luiz R, Góes Rejane M, Santos Fernanda C A, Taboga Sebastião R
Department of Structural and Functional Biology, Institute of Biology, UNICAMP, CP 6109, Campinas, São Paulo, 13084-864, Brazil.
Special Institute of Health Sciences, Medicine Course, Federal University of Goiás, Jataí, Goiás, 75804-020.
Environ Toxicol. 2017 Feb;32(2):477-489. doi: 10.1002/tox.22252. Epub 2016 Mar 4.
In rodents, the final growth and maturation of the prostate occur at puberty, a crucial period for prostate development. The present study is a serological, morphological, morphometric, and immunohistochemical analysis of the effects of exposure to ethinylestradiol (EE) (15 µg/kg/day) during puberty (EE/PUB group) on the male ventral and female prostate in senile gerbils. In the study, male and female gerbils (Meriones unguiculatus) (42 days) received by gavage 15 μg/kg/day of EE (a component of the contraceptive pill), diluted in 100 µL of Nujol® for 1 week (EE/PUB group). In the control group, males and females were not treated. Animals were killed (n = 5) after 12 months in the experimental groups. In the senile male in the EE/PUB group, we observed a reduction in testosterone levels and a decrease in the prostatic epithelial thickness, as well as in the thickness of the muscle layer. In addition, an increase in PIN multiplicity and prostatic inflammation was observed. In the senile female in the EE/PUB group, we observed increased testosterone and estradiol levels, an enhanced prostatic epithelial thickness and an increase in the thickness of the muscle layer. Immunohistochemical analysis revealed an increase in positive cells (%) for AR and PCNA in the male prostate and an increase in positive basal cells for p63 in the female prostate of the EE/PUB group. Exposure to EE during puberty resulted in an inhibitory action on the male ventral prostate and an anabolic effect on the female prostate in senile gerbils. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 477-489, 2017.
在啮齿动物中，前列腺的最终生长和成熟发生在青春期，这是前列腺发育的关键时期。本研究是一项血清学、形态学、形态计量学和免疫组织化学分析，旨在探究青春期（EE/PUB组）暴露于炔雌醇（EE）（15μg/kg/天）对老年沙鼠雄性腹侧前列腺和雌性前列腺的影响。在该研究中，雄性和雌性沙鼠（长爪沙鼠）（42日龄）通过灌胃给予15μg/kg/天的EE（避孕药的一种成分），用100μL白色凡士林稀释，持续1周（EE/PUB组）。对照组的雄性和雌性沙鼠不接受处理。12个月后处死实验组的动物（n = 5）。在EE/PUB组的老年雄性沙鼠中，我们观察到睾酮水平降低，前列腺上皮厚度以及肌肉层厚度减小。此外，还观察到前列腺上皮内瘤灶的数量增加以及前列腺炎症。在EE/PUB组的老年雌性沙鼠中，我们观察到睾酮和雌二醇水平升高，前列腺上皮厚度增加以及肌肉层厚度增加。免疫组织化学分析显示，EE/PUB组雄性前列腺中雄激素受体（AR）和增殖细胞核抗原（PCNA）的阳性细胞（%）增加；雌性前列腺中p63的阳性基底细胞增加。青春期暴露于EE对老年沙鼠的雄性腹侧前列腺产生抑制作用，对雌性前列腺产生合成代谢作用。© 2016威利期刊公司。《环境毒理学》32: 477 - 489, 2017。
