Department of Biology, Institute of Biosciences, Letters and Exact Sciences, São Paulo State University, Rua Cristóvão Colombo, 2265, São José do Rio Preto, São Paulo, Brazil.
Special Academic Unit of Health Sciences, Medicine Course, Federal University of Goiás, Rodovia BR-364 Km 195, 3800, Jataí, Goiás, Brazil.
Cell Biol Int. 2020 Jul;44(7):1467-1480. doi: 10.1002/cbin.11342. Epub 2020 Mar 28.
Prenatal and neonatal exposure to estrogenic compounds, such as ethinylestradiol (EE), promotes a variety of developmental disorders, including malformations and alterations in the morphology of glands, such as the prostate gland. Therefore, the aim of this study was to evaluate the morphological effects of neonatal exposure to EE on prostatic tissue and on the identification and quantification of gerbil gland macrophages in adult and senile Mongolian gerbils. The animals were exposed to EE (10 μg/kg/day) and to the vehicle, mineral oil (100 μL) (control group) during the first 10 days of postnatal life (lactation period). Adult gerbils were euthanized at 120 days and senile gerbils at 12 months of age. Our findings permitted verification of the presence of areas with proliferative foci in the prostate glandular portions in the adult and senile animals exposed to EE. There was also an increase in macrophages in the prostate tissue of adult and senile gerbils; these cell types alter the stromal microenvironment and possibly modify the interactions between the epithelium and stroma. Neonatal exposure to EE changes the pattern of prostatic development, leading to alterations in the arrangement of cells, including macrophages, and may be related to the onset of proliferative disorders in the prostate of adult gerbils and during aging.
产前和新生儿期暴露于雌激素化合物，如乙炔雌二醇（EE），可促进多种发育障碍，包括畸形和腺体形态改变，如前列腺。因此，本研究旨在评估新生期暴露于 EE 对前列腺组织的形态影响，以及鉴定和定量成年和老年蒙古沙鼠前列腺组织中的沙鼠腺巨噬细胞。动物在出生后第 10 天（哺乳期）接受 EE（10μg/kg/天）和矿物油（vehicle，100μL）（对照组）的处理。成年沙鼠在 120 天处死，老年沙鼠在 12 个月龄处死。我们的研究结果证实了暴露于 EE 的成年和老年动物前列腺腺体部分存在增生灶的存在。成年和老年沙鼠前列腺组织中的巨噬细胞也有所增加；这些细胞类型改变了基质微环境，并可能改变上皮细胞和基质之间的相互作用。新生期暴露于 EE 改变了前列腺的发育模式，导致细胞排列的改变，包括巨噬细胞，这可能与成年沙鼠前列腺增生性疾病的发生和衰老过程中前列腺的发生有关。
