López Sanmartín Monserrat, Power Deborah M, de la Herrán Roberto, Navas José I, Batista Frederico M
Instituto de Investigación y Formación Agraria y Pesquera (IFAPA), Centro Agua del Pino, Junta de Andalucía, Ctra. El Rompido-Punta Umbría, km 4, 21459 Cartaya, Spain.
Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
Virus Res. 2016 Jun 2;217:55-62. doi: 10.1016/j.virusres.2016.01.023. Epub 2016 Mar 3.
Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called μvar. In the present study we demonstrated that O. edulis juveniles can be infected by OsHV-1μvar when administered as an intramuscular injection. Mortality in the oysters injected with OsHV-1μvar was first detected 4 days after injection and reached 25% mortality at day 10. Moreover, the high viral load observed and the detection of viral transcripts by in situ hybridization in several tissues of dying oysters suggested that OsHV-1μvar was the cause of mortality in the O. edulis juveniles. This is therefore the first study to provide evidence about the pathogenicity of OsHV-1μvar in a species that does not belong to the Crassostrea genus. Additionally, we present a novel method to detect OsHV-1 transcripts in infected individuals' using in situ hybridization.
据报道，多种双壳贝类物种都感染了牡蛎疱疹病毒1型（OsHV-1）。自2008年以来，欧洲太平洋牡蛎稚贝的死亡率大幅上升，这与一种名为μvar的OsHV-1变种的传播有关。在本研究中，我们证明，当通过肌肉注射接种时，食用牡蛎幼体可被OsHV-1μvar感染。注射OsHV-1μvar的牡蛎在注射后第4天首次检测到死亡，到第10天死亡率达到25%。此外，在濒死牡蛎的多个组织中观察到的高病毒载量以及通过原位杂交检测到病毒转录本，表明OsHV-1μvar是食用牡蛎幼体死亡的原因。因此，这是第一项提供证据证明OsHV-1μvar对不属于太平洋牡蛎属的物种具有致病性的研究。此外，我们还提出了一种使用原位杂交检测受感染个体中OsHV-1转录本的新方法。
