Wang Na, Wang Jiamin, Liao Yuan, Shao Shijun
Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Talanta. 2016 May 1;151:1-7. doi: 10.1016/j.talanta.2016.01.011. Epub 2016 Jan 11.
A new sorbent for solid-phase extraction was synthesized by chemical immobilization of nitro-substituted tris(indolyl)methane on silica in new and green deep eutectic solvents. Elemental analysis results indicated that deep eutectic solvents could be an alternative to the traditional solvents in preparing nitro-substituted tris(indolyl)methane modified silica. Coupled with high performance liquid chromatography, the extraction performance of the sorbent was evaluated by using four organic acids as model analytes. The rebinding experiments results showed that the nitro-substituted tris(indolyl)methane modified silica sorbent had a good adsorption capacity towards the selected organic acids. Under the appropriate experimental conditions, good precision and wide linear ranges with coefficient of determination (R(2)) of higher than 0.9957 were obtained, and the limits of detection were in the range of 0.50-2.0μgL(-1) for the organic acids tested. The developed solid-phase extraction-high performance liquid chromatography-diode array detection (SPE-HPLC-DAD) method was successfully applied for the determination of organic acids in two drinking samples with recoveries ranging from 76.7% to 110.0% and 67.7% to 104.0% for all the selected organic acids, respectively.
通过在新型绿色深共熔溶剂中将硝基取代的三（吲哚基）甲烷化学固定在硅胶上，合成了一种用于固相萃取的新型吸附剂。元素分析结果表明，在制备硝基取代的三（吲哚基）甲烷改性硅胶时，深共熔溶剂可替代传统溶剂。结合高效液相色谱法，以四种有机酸为模型分析物评估了该吸附剂的萃取性能。再结合实验结果表明，硝基取代的三（吲哚基）甲烷改性硅胶吸附剂对所选有机酸具有良好的吸附能力。在适当的实验条件下，获得了良好的精密度和较宽的线性范围，测定系数（R²）高于0.9957，所测有机酸的检测限在0.50 - 2.0μg L⁻¹范围内。所建立的固相萃取 - 高效液相色谱 - 二极管阵列检测（SPE - HPLC - DAD）方法成功应用于两种饮用水样品中有机酸的测定，所有所选有机酸的回收率分别在76.7%至110.0%和67.7%至104.0%之间。
