Xie Huiting, Wu Yinglong, Huang Jing, Zeng Fang, Wu Hao, Xia Xitao, Yu Changmin, Wu Shuizhu
College of Materials Science and Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China.
College of Materials Science and Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China.
Talanta. 2016 May 1;151:8-13. doi: 10.1016/j.talanta.2016.01.015. Epub 2016 Jan 11.
Excessive amount of aluminum is detrimental to growing plants or animals and people are likely to suffer from various diseases upon long-term exposure to aluminum ions. Therefore, sensitive and selective detection of trace amounts of Al(3+) in real samples is of great importance. Herein, a ratiometric fluorescent probe for detecting aluminum ions based on pyrene-1-butyric acid (Py-L-COOH) was developed, which function via monomer/excimer conversion. In the presence of Al(3+), the original monomer-state Py-L-COOH molecules coordinate with Al(3+) and form excimer, thus changing the emission wavelength from 350-400nm to 450-500nm and achieving the ratiometric detection for Al(3+). The probe responds to Al(3+) quickly and can be operable in aqueous media with a very low detection limit of 0.29µM. This system is capable of detecting Al(3+) in real samples and shows high selectivity. Furthermore, the probe exhibits low cytotoxicity and can be used in fluorescence imaging in Hela cell lines. The approach may provide an effective simple probe for aluminum ions determination with application to real samples, as well as offering insights for designing facile ratiometric fluorescent sensors.
过量的铝对生长中的植物、动物有害，长期接触铝离子的人可能会患上各种疾病。因此，灵敏且选择性地检测实际样品中的痕量Al(3+)具有重要意义。在此，开发了一种基于芘-1-丁酸（Py-L-COOH）的用于检测铝离子的比率荧光探针，其通过单体/准分子转换起作用。在Al(3+)存在下，原始的单体态Py-L-COOH分子与Al(3+)配位并形成准分子，从而将发射波长从350 - 400nm改变为450 - 500nm，实现了对Al(3+)的比率检测。该探针对Al(3+)响应迅速，可在水性介质中操作，检测限低至0.29µM。该系统能够检测实际样品中的Al(3+)，并表现出高选择性。此外，该探针具有低细胞毒性，可用于HeLa细胞系的荧光成像。该方法可为铝离子测定提供一种有效的简单探针并应用于实际样品，同时也为设计简便的比率荧光传感器提供思路。
