Lin Shu Min, Geng Shuo, Li Na, Li Nian Bing, Luo Hong Qun
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Talanta. 2016 May 1;151:106-113. doi: 10.1016/j.talanta.2016.01.028. Epub 2016 Jan 15.
Mercury ion is one of the most hazardous metal pollutants that can cause deleterious effects on human health and the environment even at low concentrations. It is necessary to develop new mercury detection methods with high sensitivity, specificity and rapidity. In this study, a novel and green strategy for synthesizing D-penicillamine-capped copper nanoparticles (DPA-CuNPs) was successfully established by a chemical reduction method, in which D-penicillamine and ascorbic acid were used as stabilizing agent and reducing agent, respectively. The as-prepared DPA-CuNPs showed strong red fluorescence and had a large Stoke's shift (270nm). Scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, fluorescence spectroscopy, and ultraviolet-visible spectrophotometry were utilized to elucidate the possible fluorescence mechanism, which could be aggregation-induced emission effect. Based on the phenomenon that trace mercury ion can disperse the aggregated DPA-CuNPs, resulting in great fluorescence quench of the system, a sensitive and selective assay for mercury ion in aqueous solution with the DPA-CuNPs was developed. Under optimum conditions, this assay can be applied to the quantification of Hg(2+) in the 1.0-30μM concentration range and the detection limit (3σ/slope) is 32nM. The method was successfully applied to determine Hg(2+) in real water samples.
汞离子是最具危害性的金属污染物之一，即使在低浓度下也会对人类健康和环境造成有害影响。开发具有高灵敏度、特异性和快速性的新型汞检测方法很有必要。在本研究中，通过化学还原法成功建立了一种新颖的绿色合成D-青霉胺包覆铜纳米颗粒（DPA-CuNPs）的策略，其中D-青霉胺和抗坏血酸分别用作稳定剂和还原剂。所制备的DPA-CuNPs显示出强烈的红色荧光，并且具有较大的斯托克斯位移（270nm）。利用扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、荧光光谱和紫外可见分光光度法阐明了可能的荧光机制，其可能是聚集诱导发光效应。基于痕量汞离子可使聚集的DPA-CuNPs分散，导致体系荧光大幅猝灭这一现象，开发了一种用DPA-CuNPs对水溶液中汞离子进行灵敏且选择性的检测方法。在最佳条件下，该检测方法可用于定量浓度范围为1.0 - 30μM的Hg(2+)，检测限（3σ/斜率）为32nM。该方法已成功应用于实际水样中Hg(2+)的测定。
