College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Mikrochim Acta. 2020 May 14;187(6):329. doi: 10.1007/s00604-020-04271-1.
A fluorometric method for the determination of histamine has been developed based on aggregation-induced emission (AIE) effect of D-penicillamine capped copper nanoparticles (DPA-CuNPs). The fluorescent DPA-CuNPs were synthesized by a one-pot method using D-penicillamine as both reducing agent and stabilizing ligand. The size, morphology and physical chemical properties of DPA-CuNPs were examined by transmission electron microscopy (TEM), fluorescence spectroscopy, fourier transform infrared spectroscopy (FTIR) and absorption spectroscopy. The DPA-CuNPs exhibit AIE effect and show intense red fluorescence (650 nm). In the presence of histamine, DPA-CuNPs are dispersed into small homogeneous particles, causing fluorescence quenching. Based on this reaction, a histamine sensor is constructed. The fluorescence of the CuNPs solution has a good linear relationship with histamine concentration in the range 0.05 μM to 5 μM and the determination limit (3σ/slope) is 30 nM. The estimated method was successfully applied to the determination of histamine in fish, pork and red wine. Graphical abstract Schematic representation of copper nanoparticles for histamine analysis. In the presence of histamine, the strong red fluorescence of copper nanoparticles is obvious decreased through interaction of copper nanoparticles and histamine.
基于 D-青霉胺封端的铜纳米粒子（DPA-CuNPs）的聚集诱导发光（AIE）效应，建立了一种用于测定组氨酸的荧光法。通过一锅法，使用 D-青霉胺作为还原剂和稳定剂，合成了具有荧光的 DPA-CuNPs。通过透射电子显微镜（TEM）、荧光光谱、傅里叶变换红外光谱（FTIR）和吸收光谱对 DPA-CuNPs 的尺寸、形态和物理化学性质进行了研究。DPA-CuNPs 具有 AIE 效应，呈现出强烈的红色荧光（650nm）。在组氨酸存在下，DPA-CuNPs 分散成小的均匀颗粒，导致荧光猝灭。基于此反应，构建了一个组氨酸传感器。CuNPs 溶液的荧光与 0.05μM 至 5μM 范围内的组氨酸浓度呈良好的线性关系，测定限（3σ/斜率）为 30nM。该方法已成功应用于鱼、猪肉和红酒中组氨酸的测定。
