Walkiewicz A, Bulak P, Brzezińska M, Wnuk E, Bieganowski A
Institute of Agrophysics, Polish Academy of Sciences, Department of Natural Environment Biogeochemistry, Doświadczalna 4, 20-290, Lublin, Poland.
Institute of Agrophysics, Polish Academy of Sciences, Department of Natural Environment Biogeochemistry, Doświadczalna 4, 20-290, Lublin, Poland.
Environ Pollut. 2016 Jun;213:403-411. doi: 10.1016/j.envpol.2016.02.048. Epub 2016 Mar 3.
Soils are the largest terrestrial sink for methane (CH4). However, heavy metals may exert toxicity to soil microorganisms, including methanotrophic bacteria. We tested the effect of lead (Pb), zinc (Zn) and nickel (Ni) on CH4 oxidation (1% v/v) and dehydrogenase activity, an index of the activity of the total soil microbial community in Mollic Gleysol soil in oxic and hypoxic conditions (oxia and hypoxia, 20% and 10% v/v O2, respectively). Metals were added in doses corresponding to the amounts permitted of Pb, Zn, Ni in agricultural soils (60, 120, 35 mg kg(-1), respectively), and half and double of these doses. Relatively low metal contents and O2 status reflect the conditions of most agricultural soils of temperate regions. Methane consumption showed high tolerance to heavy metals. The effect of O2 status was stronger than that of metals. CH4 consumption was enhanced under hypoxia, where both the start and the completion of the control and contaminated treatment were faster than under oxic conditions. Dehydrogenase activity, showed higher sensitivity to the contamination (except for low Ni dose), with a stronger effect of heavy metals, than that of the O2 status.
土壤是甲烷（CH₄）最大的陆地汇。然而，重金属可能会对包括甲烷氧化菌在内的土壤微生物产生毒性。我们测试了铅（Pb）、锌（Zn）和镍（Ni）对CH₄氧化（体积分数1%）以及脱氢酶活性的影响，脱氢酶活性是衡量在有氧和缺氧条件下（分别为20%和10%体积分数的氧气，即好氧和缺氧）潜育性灰壤土中土壤微生物群落总活性的一个指标。添加的金属剂量分别对应于农业土壤中允许的Pb、Zn、Ni含量（分别为60、120、35毫克/千克），以及这些剂量的一半和两倍。相对较低的金属含量和氧气状况反映了温带地区大多数农业土壤的条件。甲烷消耗对重金属表现出较高的耐受性。氧气状况的影响比金属的影响更强。在缺氧条件下，CH₄消耗增加，对照和污染处理的开始和完成速度均比有氧条件下更快。脱氢酶活性对污染（低镍剂量除外）表现出更高的敏感性，重金属的影响比氧气状况的影响更强。
