[高丁十字花科六个物种的ISSR扩增结果分析]

[Analysis of ISSR Amplification Results of Six Species in Sect. Cruciata Gaudin].

作者信息

Zhou Wen-ping, Zhang Hui-juan, Wang Ya-fei, Wang Zhe-zhi, Cao Xiao-yan

出版信息

Zhong Yao Cai. 2015 Jul;38(7):1375-8.

Abstract

OBJECTIVE

To identify and analyze the genetic relationship of four species of Gentiana (G. macrophylla, G. straminea, G. dahurica and G. crassicaulis) recorded as Gentianae Macrophyllae Radix in the Chinese Pharmacopoeia, and two other Gentiana species (G. officinalis and G. siphonantha) often used as substitutes by ISSR, in order to estimate the reasonability of G. officinalis and G. siphonantha used as substitutes from the DNA level.

METHODS

Eight primers ivere screened to amplify all the samples and agarose gel electrophoresis were analyzed. NTSYSpc-2. 10E software was used to calculate similarity coefficient and draw dendrogram. Results: Nine characteristic bands were found in different species on the ISSR fingerprints and which could be used to identify five species except G. dahurica. The substitute G. officinalis firstly clustered with G. dahurica and G. siphonantha showed closer genetic relationship with G. straminea and G. dahurica. G. crassicaulis showed a far genetic relationship with the other five species.

CONCLUSION

The dendrogram based on the ISSR data supports that G. officinalis and G. siphonantha can be used as substitutes of Gentianae Macrophyllae Radix.

摘要

目的

利用ISSR技术对《中国药典》收载的作秦艽入药的4个龙胆属物种（大叶秦艽、粗茎秦艽、麻花秦艽和小秦艽）及2种常见的代用品（管花秦艽和西藏秦艽）进行亲缘关系鉴定与分析，从DNA水平探讨管花秦艽和西藏秦艽作为秦艽代用品的合理性。

方法

筛选8条引物对所有样品进行扩增，分析琼脂糖凝胶电泳结果，采用NTSYSpc - 2.10E软件计算相似系数并绘制聚类图。结果：在ISSR指纹图谱上，各物种共出现9条特征条带，可用于鉴别除麻花秦艽外的5个物种。代用品管花秦艽首先与麻花秦艽聚类，西藏秦艽与粗茎秦艽和麻花秦艽亲缘关系较近，小秦艽与其他5个物种亲缘关系较远。