Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Phytochem Anal. 2010 Jul-Aug;21(4):348-54. doi: 10.1002/pca.1206.
Radix Gentianae Macrophyllae, a traditional Chinese medicine, has been frequently used to dispel rheumatism and ease pain. There are four species of Gentiana (G. macrophylla, G. straminea, G. dahurica and G. crassicaulis) recorded as herbal drugs in the Chinese Pharmacopoeia and two other Gentiana species (G. officinalis and G. siphonantha) are often used as substitutes. Currently, the LC fingerprint comparison among different species and evidence for the equivalent application of these herbs are lacking.
To develop an HPLC method for the simultaneous determination of four iridoid and secoiridoid glycosides and a comparative study of six species of Gentiana.
HPLC analysis was performed on a C(18) column (Phenomenex, 150 x 4.6 mm, 5 microm particle size) with gradient elution using 0.4% aqueous phosphoric acid and methanol at 242 nm.
The proposed method was precise, accurate and sensitive enough for simultaneous quantitative evaluation of four iridoid and secoiridoid glycosides (loganic acid, swertiamarin, gentiopicroside and sweroside) in the six species of Gentiana. Contents of the four marker compounds varied from each other even among the samples from the same species and the LC chromatograms of the six species of Gentiana showed high similarities.
he close similarity of LC chromatograms and chemical composition of the four genuine Gentiana species explain their popular usage as Radix Gentianae Macrophyllae in Chinese medicine. By comparing the four genuine Gentiana species, it is suggested that the two substitutes could be used as Radix Gentianae Macrophyllae to relieve the scarcity of resources.
獐牙菜，一种传统的中药，常用于祛风湿、止痛。中国药典收录了獐牙菜属的四个种（如大叶獐牙菜、扁茎獐牙菜、东北獐牙菜和川西獐牙菜）作为草药，另有两个种（如秦艽和华獐牙菜）也常被用作替代品。目前，不同种獐牙菜之间的 LC 指纹图谱比较以及这些草药等效应用的证据尚缺乏。
建立一种同时测定四种环烯醚萜和裂环环烯醚萜苷类成分的 HPLC 方法，并对六种獐牙菜属植物进行比较研究。
采用高效液相色谱法，在 Phenomenex C18 柱（150×4.6mm，5μm）上，以 0.4%磷酸水溶液-甲醇为流动相进行梯度洗脱，检测波长为 242nm。
该方法精确、准确、灵敏，可用于同时定量评价六种獐牙菜属植物中四种环烯醚萜和裂环环烯醚萜苷类成分（獐牙菜苦苷、当药苦苷、龙胆苦苷和獐牙菜苦苷甲酯）。即使是同一物种的样品，四种标记化合物的含量也存在差异，而六种獐牙菜属植物的 LC 图谱显示出高度的相似性。
四种正品獐牙菜属植物的 LC 图谱和化学成分的高度相似性解释了它们在中国医学中作为獐牙菜的广泛应用。通过比较四种正品獐牙菜属植物，建议将两种替代品作为獐牙菜来缓解资源短缺。
