Endo Hirotoshi, Yoshida Megumi, Uji Toshiki, Saga Naotsune, Inoue Koji, Nagasawa Hiromichi
Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Japan.
Graduate School of Agricultural and Life Sciences, the University of Tokyo, Japan.
Sci Rep. 2016 Mar 7;6:22252. doi: 10.1038/srep22252.
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.
在三大主要海洋微藻类群中，甲藻和硅藻能够进行遗传转化；然而，迄今为止尚未建立针对定鞭藻的转化方法。在此，我们报告了通过聚乙二醇（PEG）介导细菌潮霉素B抗性基因的转移，首次实现了球石藻——卡氏普列藻（Pleurochrysis carterae）的稳定遗传转化。结合新型瞬时绿色荧光蛋白（GFP）表达系统，这种方法应有助于该门（定鞭藻门）基于分子的进一步研究。
