Borges Wesley da Silva, Araújo Breno Severiano Alves, Moura Lucas Gomes, Coutinho Filho Ubirajara, de Resende Miriam Maria, Cardoso Vicelma Luiz
Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil.
Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil.
J Environ Manage. 2016 May 15;173:134-40. doi: 10.1016/j.jenvman.2015.11.015. Epub 2016 Mar 2.
The objective of this study was to evaluate the bio-oil production and the organic load removal using the microalga Scenedesmus sp. The cultivation was carried out in reactors with a total volume of 3 L and 0.7 vvm aeration, with illumination in photoperiods of 12 h light/12 h dark for 12 days. The following sugar concentrations were tested: 2.5, 5.0 and 10 g/L of glucose, lactose, fructose and galactose with 10% inoculum volume. After experiments were performed with cheese whey in natura and cheese whey permeate with different lactose concentrations (1.5, 2.5, 3.5 and 5.0 g/L). In these experiments the inoculum concentrations were 10, 15, 20 and 30% (v/v). The results showed that this microalga was effective for the production of lipids when it was cultivated in medium with cheese whey in natura with 2.5 g/L of lactose and 20% inoculum (v/v). Using cheese whey in natura at the concentration of 3.5 g/L of lactose and 30% (v/v) of inoculum obtained 77.9% of TOC removal and 38.447 mg of TOC removed/mg oil produced. It was also observed that when there is increased production of bio-oil, there is less removal of organic matter. The addition of glucose, fructose or galactose in the medium did not enhance the production of bio-oil by Scenedesmus sp. when compared to lactose, but increased the organic matter removal.
本研究的目的是评估使用微藻斜生栅藻生产生物油以及去除有机负荷的情况。培养在总体积为3 L的反应器中进行，通气量为0.7 vvm，光照周期为12小时光照/12小时黑暗，持续12天。测试了以下糖浓度：葡萄糖、乳糖、果糖和半乳糖的浓度分别为2.5、5.0和10 g/L，接种量为10%。之后用天然奶酪乳清和不同乳糖浓度（1.5、2.5、3.5和5.0 g/L）的奶酪乳清渗透液进行实验。在这些实验中，接种浓度为10%、15%、20%和30%（v/v）。结果表明，当这种微藻在含有2.5 g/L乳糖和20%接种量（v/v）的天然奶酪乳清培养基中培养时，对脂质生产有效。使用浓度为3.5 g/L乳糖和30%（v/v）接种量的天然奶酪乳清，TOC去除率达到77.9%，每毫克生物油产生去除38.447毫克TOC。还观察到，当生物油产量增加时，有机物去除量减少。与乳糖相比，在培养基中添加葡萄糖、果糖或半乳糖并没有提高斜生栅藻的生物油产量，但增加了有机物去除量。
