Calzia R, Marazzi M G, Campelli A, Piscopo R, Dorati M, Puppo F, Borgiani L, Canepa M
Dept. of Internal Medicine, University of Genoa, Italy.
Adv Exp Med Biol. 1989;257:225-35. doi: 10.1007/978-1-4684-5712-4_22.
Studies on the quantitative expression of the Major Histocompatibility Complex (MCH) in hepatocytes chronically infected by Hepatitis B Virus (HBV) report that an increased expression of these antigens could be related to a good immunological response. In the present work we analyze the expression of the MCH antigens in cryostatic sections of liver biopsies taken from subjects (19 children) with various forms of HBsAg positive chronic hepatitis. A high expression of HLA class I antigens and a high degree of hepatocyte necrosis was evident in Chronic Active Hepatitis (CAH) and Chronic Lobular Hepatitis (CLH). On the contrary, subjects with histological diagnosis of Chronic Persistent Hepatitis (CPH) showed a low expression of such antigens. There was however, the difference that in subjects with high hepatic cytolysis and high expression of HLA class I antigens, serum HBV-DNA was clearly present in almost all the cases with CAH, but not detectable in all cases with CLH. The expression of HLA class II antigens and of Beta2 microglobulin was the same in all 19 cases. All cases with HBV-DNA positivity with high class I antigen expression had active hepatitis which seems to suggest that all attempts at viral clearance on the part of the immune system have been in vain. We hope our paper will be an additional parameter for evaluating the course of hepatitis during Interferon treatment.
关于乙型肝炎病毒（HBV）慢性感染的肝细胞中主要组织相容性复合体（MCH）定量表达的研究报告称，这些抗原表达的增加可能与良好的免疫反应有关。在本研究中，我们分析了从患有各种形式HBsAg阳性慢性肝炎的受试者（19名儿童）肝脏活检的冷冻切片中MCH抗原的表达情况。在慢性活动性肝炎（CAH）和慢性小叶性肝炎（CLH）中，HLA I类抗原的高表达和高度的肝细胞坏死很明显。相反，组织学诊断为慢性持续性肝炎（CPH）的受试者显示出此类抗原的低表达。然而，存在这样的差异：在肝细胞溶解程度高且HLA I类抗原高表达的受试者中，几乎所有CAH病例的血清HBV-DNA均明显存在，但并非所有CLH病例均可检测到。在所有19例病例中，HLA II类抗原和β2微球蛋白的表达相同。所有HBV-DNA阳性且I类抗原高表达的病例均患有活动性肝炎，这似乎表明免疫系统清除病毒的所有尝试均徒劳无功。我们希望我们的论文将成为评估干扰素治疗期间肝炎病程的一个额外参数。
