Illangasekera Yasitha A, Kumarasiri Ranjith P V, Fernando Devaka J, Dalton Caroline F
Department of Pharmacology, Faculty of Medicine, University of Peradeniya, Sri Lanka; Biomolecular Sciences Research Centre, Faculty of Health and Wellbeing, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom.
Department of Community Medicine, Faculty of Medicine, University of Peradeniya, Sri Lanka.
Obes Res Clin Pract. 2016 Sep;10 Suppl 1:S117-S124. doi: 10.1016/j.orcp.2016.02.003. Epub 2016 Mar 3.
To investigate the association between the fat mass and obesity related (FTO) gene rs9939609 and near melanocortin-4-receptor (MC4R) gene rs17782313 polymorphisms with obesity measures and metabolic parameters in urban and rural dwelling Sri Lankans.
535 subjects (60.9% female) from the general adult population (ages 18-70 years) representative of both urban (28.4%) and rural areas of residence were recruited by multi-stage random sampling. Body mass index (BMI), waist circumference (WC) and waist-to-hip ratio (WHR) was obtained by standard methods. DNA extracted from whole blood was genotyped using real-time PCR.
The FTO risk genotypes (AA+AT) were associated with higher BMI (p=0.03) and WC (p=0.05) measures as well as categorical obesity (BMI ≥27.5kgm definition) (OR 1.69 95% CI 1.11-2.56, p=0.01). The near MC4R risk genotypes (CC+CT) were associated with greater BMI (p=0.03) as well as categorical obesity (BMI ≥25kgm definition) (OR 1.57 95% CI 1.11-2.22, p=0.01). In addition the MC4R risk genotype carriers (CC+CT) had significantly higher fasting blood sugar (FBS) levels compared to the 'TT' genotype carriers independent of BMI (p=0.05). Urban living was associated with significantly greater BMI values for FTO risk genotypes compared to rural living (p=0.02).
FTO and near MC4R variants are associated with obesity measures in Sri Lankan populations whilst urban living accentuates the obesogenic effect of the FTO polymorphism.
研究脂肪量和肥胖相关（FTO）基因rs9939609以及近黑皮质素-4-受体（MC4R）基因rs17782313多态性与斯里兰卡城乡居民肥胖指标及代谢参数之间的关联。
通过多阶段随机抽样，从代表城市（28.4%）和农村地区的18至70岁普通成年人群中招募了535名受试者（女性占60.9%）。采用标准方法获取体重指数（BMI）、腰围（WC）和腰臀比（WHR）。从全血中提取DNA，使用实时PCR进行基因分型。
FTO风险基因型（AA + AT）与较高的BMI（p = 0.03）和WC（p = 0.05）指标以及分类肥胖（BMI≥27.5kg/m定义）相关（比值比1.69，95%置信区间1.11 - 2.56，p = 0.01）。近MC4R风险基因型（CC + CT）与更高的BMI（p = 0.03）以及分类肥胖（BMI≥25kg/m定义）相关（比值比1.57，95%置信区间1.11 - 2.22，p = 0.01）。此外，与“TT”基因型携带者相比，MC4R风险基因型携带者（CC + CT）的空腹血糖（FBS）水平显著更高，且与BMI无关（p = 0.05）。与农村生活相比，城市生活中FTO风险基因型的BMI值显著更高（p = 0.02）。
FTO和近MC4R变异与斯里兰卡人群的肥胖指标相关，而城市生活加剧了FTO多态性的致肥胖效应。
