National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy.
Food Chem. 2016 Jul 15;203:375-378. doi: 10.1016/j.foodchem.2016.02.095. Epub 2016 Feb 15.
The authentication of food components is one of the key issues in food safety. Similarly taxonomy, population and conservation genetics as well as food web structure analysis, also rely on genetic analyses including the DNA barcoding technology. In this scenario we developed a fast DNA extraction method without any purification step from fresh and processed seafood, suitable for any PCR analysis. The protocol allows the fast DNA amplification from any sample, including fresh, stored and processed seafood and from any waste of industrial fish processing, independently of the sample storage method. Therefore, this procedure is particularly suitable for the fast processing of samples and to carry out investigations for the authentication of seafood by means of DNA analysis.
食品成分的鉴定是食品安全的关键问题之一。同样，分类学、种群和保护遗传学以及食物网结构分析也依赖于包括 DNA 条码技术在内的遗传分析。在此背景下，我们开发了一种无需任何纯化步骤即可从新鲜和加工海产品中提取 DNA 的快速方法，适用于任何 PCR 分析。该方案允许从任何样品（包括新鲜、储存和加工的海产品以及工业鱼类加工的任何废物）中快速进行 DNA 扩增，而与样品储存方法无关。因此，该程序特别适合快速处理样品，并通过 DNA 分析进行海鲜鉴定的调查。
