Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Dipartimento di Scienze AgroAlimentari, Ambientali e Animali, Università di Udine, 33100 Udine, Italy.
Sensors (Basel). 2019 Mar 4;19(5):1100. doi: 10.3390/s19051100.
Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.
食源性致病菌是食品安全的一个关键问题。传统的诊断方法耗时且只能针对已生产的食品进行。将分子方法、生物传感器、微流控和纳米材料集成的即时诊断设备领域的进展为快速、低成本、高灵敏度和特异性地检测病原体提供了新途径。这些对食品的分析和筛选可以在生产的所有阶段进行。本综述介绍了近年来在陆基领域即时诊断方面取得的主要进展，并探讨了在食品工业中更广泛接受便携式设备所面临的当前挑战。特别强调了测试核酸的方法、便携式核酸提取和扩增的方案，以及低成本检测和读出信号放大的手段。
