Dong Hao, Li Xuemin, Xue Changhu, Mao Xiangzhao
College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Biotechnol Prog. 2016 May;32(3):649-56. doi: 10.1002/btpr.2258. Epub 2016 May 17.
Natural astaxanthin (Ax) is an additive that is widely used because of its beneficial biochemical functions. However, the methods used to produce free Ax have drawbacks. Chemical saponification methods produce several by-products, and lipase-catalyzed hydrolysis methods are not cost effective. In this study, a bacterial strain of Stenotrophomonas sp. was selected to enzymatically catalyze the saponification of Ax esters to produce free all-trans-Ax. Through single-factor experiments and a Box-Behnken design, the optimal fermentation conditions were determined as follows: a seed culture age of 37.79 h, an inoculum concentration of 5.92%, and an initial broth pH of 6.80. Under these conditions, a fermentation curve was drawn, and the optimal fermentation time was shown to be 60 h. At 60 h, the degradation rate of the Ax esters was 98.08%, and the yield of free all-trans-Ax was 50.130 μg/mL. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:649-656, 2016.
天然虾青素（Ax）是一种因其有益的生化功能而被广泛使用的添加剂。然而，用于生产游离虾青素的方法存在缺陷。化学皂化法会产生多种副产物，而脂肪酶催化水解法不具有成本效益。在本研究中，一株嗜麦芽窄食单胞菌被选用于酶促催化虾青素酯的皂化反应以生产游离全反式虾青素。通过单因素实验和Box-Behnken设计，确定了最佳发酵条件如下：种子培养龄为37.79小时，接种浓度为5.92%，初始发酵液pH值为6.80。在这些条件下绘制了发酵曲线，结果表明最佳发酵时间为60小时。在60小时时，虾青素酯的降解率为98.08%，游离全反式虾青素的产量为50.130μg/mL。©2016美国化学工程师学会生物技术进展，32:649-656，2016年。
