Walker-Caprioglio H M, MacKenzie J M, Parks L W
Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
Antimicrob Agents Chemother. 1989 Dec;33(12):2092-5. doi: 10.1128/AAC.33.12.2092.
Polyclonal antibodies elicited by injection into rabbits of a nystatin-bovine serum albumin conjugate were reactive with both nystatin and amphotericin B. Upon labeling of polyene-treated Saccharomyces cerevisiae sterol auxotrophs grown on various sterols, nystatin reacted specifically with ergosterol, while amphotericin B did not react preferentially with ergosterol, cholesterol, or cholestanol. Time course labeling experiments demonstrated the rate of ergosterol transport into cholesterol-grown cells.
通过向兔子注射制霉菌素 - 牛血清白蛋白偶联物引发的多克隆抗体与制霉菌素和两性霉素B均有反应。在用多烯处理并在各种固醇上生长的酿酒酵母固醇营养缺陷型进行标记时，制霉菌素与麦角固醇特异性反应，而两性霉素B与麦角固醇、胆固醇或胆甾烷醇均无优先反应。时间进程标记实验证明了麦角固醇转运到以胆固醇为生长底物的细胞中的速率。
