诱导用于免疫测定的两性霉素B特异性抗体。
Induction of amphotericin B-specific antibodies for use in immunoassays.
作者信息
Little J R, Little K D, Plut E, Koldin M, Kobayashi G S
出版信息
Antimicrob Agents Chemother. 1984 Dec;26(6):824-8. doi: 10.1128/AAC.26.6.824.
Abstract
High-titered antisera specific for amphotericin B (AmB) were induced by immunization with a protein conjugate of the D-lysyl AmB methyl ester. These polyclonal anti-AmB antibodies reacted preferentially with AmB or the AmB methyl ester and discriminated sharply between nystatin and AmB. A solid-phase radioimmunoassay was developed with radioiodinated immunoglobulin G fractions derived from the anti-AmB antisera. This assay was capable of detecting AmB in the sera in the same concentration range that is regularly achieved during AmB treatment of systemic fungal infections. This study demonstrated the feasibility of immunoassays in measuring the concentration of AmB in blood and tissue fluids.
摘要
用两性霉素B（AmB）的D-赖氨酰甲酯蛋白偶联物免疫诱导出了高滴度的抗AmB特异性抗血清。这些多克隆抗AmB抗体优先与AmB或AmB甲酯反应，并能清晰地区分制霉菌素和AmB。利用从抗AmB抗血清中获得的放射性碘化免疫球蛋白G组分开发了一种固相放射免疫测定法。该测定法能够检测血清中的AmB，其浓度范围与全身性真菌感染患者接受AmB治疗期间通常达到的浓度范围相同。本研究证明了免疫测定法在测量血液和组织液中AmB浓度方面的可行性。
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