珍稀特有灌木红毛四数花（杜英科）13个微卫星的分离与特征分析
Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae).
作者信息
Anthony Janet M, Allcock Richard J N, Krauss Siegfried L
机构信息
Kings Park and Botanic Garden, Botanic Garden and Parks Authority, Fraser Avenue, West Perth, 6005 Western Australia, Australia; School of Plant Biology, The University of Western Australia, Nedlands, 6009 Western Australia, Australia.
School of Pathology and Laboratory Medicine, Lotterywest State Biomedical Facility Genomics, University of Western Australia, Crawley, Western Australia 6009, Australia.
出版信息
Appl Plant Sci. 2016 Feb 9;4(2). doi: 10.3732/apps.1500102. eCollection 2016 Feb.
PREMISE OF THE STUDY
Microsatellite markers were developed for the rare Tetratheca erubescens (Elaeocarpaceae) to assess genetic diversity and spatial structuring.
METHODS AND RESULTS
We generated ca. 2.7 million sequence reads using a Personal Genome Machine (PGM) semiconductor sequencer. Using the QDD pipeline, we designed primers for >12,000 sequences with PCR product lengths of 80-480 bp. From these, 30 primer pairs were selected and screened using PCR, from which 11 loci were found to be polymorphic and amplified reliably. For a sample of 95 plants from three populations, the number of alleles observed for these 11 loci ranged from two to seven and expected heterozygosity ranged from 0.06 to 0.72. No consistent evidence for null alleles or departure from Hardy-Weinberg equilibrium was found for any of the 11 loci.
CONCLUSIONS
These markers will enable the quantification of genetic impact of proposed mining activities on the narrow endemic T. erubescens.
研究前提
为珍稀植物红毛肖槿（杜英科）开发微卫星标记，以评估其遗传多样性和空间结构。
方法与结果
我们使用个人基因组测序仪（PGM）半导体测序仪生成了约270万个序列读数。使用QDD流程，我们为超过12000个序列设计了引物，PCR产物长度为80 - 480 bp。从中选择了30对引物并通过PCR进行筛选，发现其中11个位点具有多态性且扩增可靠。对于来自三个种群的95株植物样本，这11个位点观察到的等位基因数从2到7不等，预期杂合度从0.06到0.72不等。在这11个位点中，没有发现无效等位基因或偏离哈迪 - 温伯格平衡的一致证据。
结论
这些标记将能够量化拟议的采矿活动对狭域特有种红毛肖槿的遗传影响。
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