从 Tetratheca ericifolia（杜英科）中分离和表征多态性微卫星位点。

Isolation and characterization of polymorphic microsatellite loci from Tetratheca ericifolia (Elaeocarpaceae).

机构信息

National Herbarium of New South Wales, Botanic Gardens Trust, Mrs Macquaries Road, Sydney, NSW 2000, Australia, Ecosystem Management, University of New England, Armidale, NSW 2351, Australia.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):867-9. doi: 10.1111/j.1755-0998.2008.02093.x.

DOI:10.1111/j.1755-0998.2008.02093.x

PMID:21585915

Abstract

We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)(n) -enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group.

摘要

我们从 Tetratheca ericifolia 的（AG）（n）富集文库中鉴定出 11 个信息丰富的微卫星位点。使用这些位于两个群体（每个群体 16 个个体）的 32 个个体上的位点，我们在每个位点检测到平均 11.3 个等位基因（范围为 5 到 21，平均预期杂合度为 0.728），其中 56%是一个群体或另一个群体所特有的。在相同的反应条件下，这七个到十二个位点可以在 Tetratheca 的另外 12 种物种中扩增。这些标记将成为该澳大利亚特有群体进化研究的有用工具。