Yan Youyou, Zhang Bin, Liu Ning, Qi Chao, Xiao Yanlong, Tian Xin, Li Tianyi, Liu Bin
Department of Cardiology, Second Hospital of Jilin University, No. 218 Ziqiang Street, Changchun 130041, China.
Biomed Res Int. 2016;2016:8079372. doi: 10.1155/2016/8079372. Epub 2016 Feb 1.
Acute myocardial infarction (AMI) is the most serious cardiovascular disease with high morbidity and mortality. Recent studies have showed that long noncoding RNAs (lnc RNA) play important roles in pathophysiology of cardiovascular diseases, but the investigations are still in their infancy. An lnc RNA named urothelial carcinoma-associated 1 (UCA1) is found in tumors such as bladder cancers and lung cancer. And the UCA1 could be as a predictive biomarker for bladder cancer in urine samples or lung cancer in plasma, respectively. In normal states, UCA1 is specifically expressed in heart of adult, indicating that UCA1 might be as a biomarker for heart diseases such as AMI. To test the speculation, we detect the level of UCA1 in plasma of AMI patients and health control using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In addition, we also test the level of miR-1 as it is reported to regulate the expression of UCA1. The results show that the level of plasma UCA1 is decreased at the early state of AMI patients and increased at day 3 after AMI. In addition, the UCA1 alteration is inversely associated with the expression of miR-1. These findings indicate that the circulating UCA1 could be used as a promising novel biomarker for the diagnosis and/or prognosis of AMI.
急性心肌梗死（AMI）是最严重的心血管疾病，具有高发病率和死亡率。最近的研究表明，长链非编码RNA（lncRNA）在心血管疾病的病理生理学中发挥重要作用，但相关研究仍处于起步阶段。一种名为尿路上皮癌相关1（UCA1）的lncRNA在膀胱癌和肺癌等肿瘤中被发现。并且UCA1可分别作为尿液样本中膀胱癌或血浆中肺癌的预测生物标志物。在正常状态下，UCA1在成年心脏中特异性表达，表明UCA1可能作为诸如AMI等心脏病的生物标志物。为了验证这一推测，我们使用定量逆转录-聚合酶链反应（qRT-PCR）检测AMI患者和健康对照者血浆中UCA1的水平。此外，我们还检测了miR-1的水平，因为据报道它可调节UCA1的表达。结果显示，AMI患者早期血浆UCA1水平降低，AMI后第3天升高。此外，UCA1的改变与miR-1的表达呈负相关。这些发现表明，循环UCA1可作为一种有前景的新型生物标志物用于AMI的诊断和/或预后评估。
