Li Yifan, Wang Tiantian, Li Yuchi, Chen Duqun, Yu Zuhu, Jin Lu, Ni Liangchao, Yang Shangqi, Mao Xiangming, Gui Yaoting, Lai Yongqing
Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
Department of Urology, Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Mol Med Rep. 2016 Apr;13(4):3326-34. doi: 10.3892/mmr.2016.4894. Epub 2016 Feb 16.
Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults, which is associated with poor prognosis and high recurrence. Long non‑coding RNAs (lncRNAs) have been reported to be dysregulated in cancer and to be important in the regulation of carcinogenesis, thus suggesting that this class of molecules may be used as biomarkers in cancer. The lncRNA urothelial carcinoma associated 1 (UCA1) has been observed to be upregulated and to function as an oncogene in certain types of cancer; however, the role of UCA1 in RCC remains to be elucidated. The present study aimed to determine the expression and function of UCA1 in RCC. Quantitative polymerase chain reaction (qPCR) was used to determine the expression levels of UCA1 in 46 paired RCC and adjacent normal tissue samples. Furthermore, qPCR was used to determine the expression levels of UCA1 in four RCC cell lines compared with the human embryonic kidney 293T cell line. The impact of UCA1 on cell migration, proliferation and apoptosis was investigated by wound scratch assay, MTT and flow cytometry, respectively. The results of the present study demonstrated that UCA1 expression levels were significantly increased in RCC tissues and cells, as compared with the controls. Ectopic expression and gene silencing of UCA1 in RCC cell lines exerted opposite effects on cellular proliferation, migration and apoptosis, and the results suggested that UCA1 may function as an oncogene in RCC. These results indicated that UCA1 may be considered as a promising biomarker for diagnosis, and a therapeutic target in RCC. Further research is required to elucidate the role and target genes of UCA1 in RCC.
肾细胞癌（RCC）是成人中最常见的肾癌类型，其预后较差且复发率高。据报道，长链非编码RNA（lncRNA）在癌症中表达失调，并且在肿瘤发生的调控中起重要作用，因此表明这类分子可能用作癌症的生物标志物。已观察到lncRNA尿路上皮癌相关1（UCA1）在某些类型的癌症中上调并作为癌基因发挥作用；然而，UCA1在RCC中的作用仍有待阐明。本研究旨在确定UCA1在RCC中的表达和功能。采用定量聚合酶链反应（qPCR）测定46对RCC及其癌旁正常组织样本中UCA1的表达水平。此外，与人类胚胎肾293T细胞系相比，采用qPCR测定UCA1在四种RCC细胞系中的表达水平。分别通过伤口划痕试验、MTT法和流式细胞术研究UCA1对细胞迁移、增殖和凋亡的影响。本研究结果表明，与对照组相比，RCC组织和细胞中UCA1表达水平显著升高。RCC细胞系中UCA1的异位表达和基因沉默对细胞增殖、迁移和凋亡产生相反的影响，结果表明UCA1可能在RCC中作为癌基因发挥作用。这些结果表明，UCA1可被视为一种有前景的RCC诊断生物标志物和治疗靶点。需要进一步研究以阐明UCA1在RCC中的作用和靶基因。
